ROR1 antibody
Principal name
ROR1 antibody
Alternative names for ROR1 antibody
NTRKR1, Tyrosine-protein kinase transmembrane receptor ROR1
Gene ID
4919 (ROR1)
Ncbi ID
9606, NP_005003.2, NM_005012, NM_001083592, NP_005003, NP_001077061
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of ROR1 antibody
ROR1, a type I membrane protein, is a receptor protein tyrosine kinase that modulates neurite growth in the central nervous system. The ROR-family receptor tyrosine kinases consist of two structurally related proteins, ROR1 and ROR2. These proteins are characterized by having intracellular tyrosine kinase domains, which are highly related to Trk-family kinases, extracellular Frizzled-like cysteine-rich domains (CRDs) and Kringle domains. The ROR family members are highly conserved among species, such as C. elegans, Drosophila, Xenopus and mammals. ROR1 and ROR2 are both involved in organogenesis with particular emphasis in neuronal differentiation. Increased expression of ROR1 in acute lymphoblastic leukemias (ALLs) as well as chronic lymphocytic leukemias (CLLs) implicate this protein as a potential tool for targeted immunotherapy in these diseases.
ROR1 monoclonal antibody (M01), clone 2F8. Western Blot analysis of ROR1 expression in NIH/3T3(Cat # L018V1 ).
ROR1 monoclonal antibody (M01), clone 2F8. Western Blot analysis of ROR1 expression in MES-SA/Dx5 ( Cat # L021V1 ).
ROR1 monoclonal antibody (M01), clone 2F8. Western Blot analysis of ROR1 expression in HepG2 ( Cat # L019V1 ).
ROR1 monoclonal antibody (M01), clone 2F8. Western Blot analysis of ROR1 expression in PC-12(Cat # L012V1 ).
Western blot analysis of ROR1 ( arrow ) using rabbit ROR1 polyclonal antibody ( Cat # PAB3384 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the ROR1 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of ROR1 (arrow) using rabbit polyclonal ROR1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ROR1 gene (Lane 2).
ROR1 monoclonal antibody (M02), clone 1B4. Western Blot analysis of ROR1 expression in Hela NE(Cat # L013V3 ).
Confocal Immunofluorescence analysis of HEK293 cells trasfected with extracellular ROR1 (aa30-406)-hIgGFc using ROR1 antibody Cat.-No AM06399SU-N (green). Blue: DRAQ5 fluorescent DNA dye.
Western blot analysis using ROR1 antibody Cat.-No AM06399SU-N against extracellular domain of human ROR1 (aa30-423).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROR1 (RC214967, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROR1.
Western blot analysis of ROR1 (arrow) using rabbit polyclonal ROR1 Antibody (Cat.#TA302193).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ROR1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the ROR1 antibody (Cat.#TA302193), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of NCI-H460 cells using ROR1 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-ROR1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.
Anti-ROR1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.
Host: Rabbit; Target Name: ROR1; Sample Tissue: HepG2 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
TA348984 (5 ug/ml) staining of paraffin embedded Human Breast cancer. Heated antigen retrieval with citrate buffer pH 6, DAB-staining.
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP314967 | ROR1 (transcript variant 1) |
||||
| Recombinant protein of human receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$788.00
|
|
| OriGene Technologies, Inc. | |||||
| TP324765 | ROR1 (transcript variant 2) |
||||
| Recombinant protein of human receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP700158 | ROR1 (transcript variant 1) |
||||
| Purified recombinant protein of Homo sapiens receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 1, residue 30-406 aa, expressed in HEK293 cells. | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP700159 | ROR1 (C-term DDK/His tag, transcript variant 2) |
||||
| Purified recombinant protein of Human receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 2, with C-terminal DDK/His tag, expressed in human cells | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP700184 | ROR1 (transcript variant 1) |
||||
| Purified recombinant protein of Homo sapiens receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 1, residue 30-406 aa, expressed in HEK293 cells, with Fc tag | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||