RNF144B antibody
Principal name
RNF144B antibody
Alternative names for RNF144B antibody
RING finger protein 144B, IBRDC2, P53RFP, E3 ubiquitin-protein ligase RNF144B, IBR domain-containing protein 2, p53-inducible RING finger protein
Ncbi ID
9606, NP_877434, NP_877434.2, NM_182757, NP_001164114.1, NM_001170643
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F)
Background of RNF144B antibody
- Kit Component:
- - KN209302G1, RNF144B gRNA vector 1 in pCas-Guide vector
- KN209302G2, RNF144B gRNA vector 2 in pCas-Guide vector
- KN209302D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to IBRDC2 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 1.2 ug/ml]
Immunofluorescence of monoclonal antibody to IBRDC2 on HeLa cell. [antibody concentration 10 ug/ml]
IBRDC2 monoclonal antibody (M02), clone 4F1 Western Blot analysis of IBRDC2 expression in IMR-32 ( Cat # L008V1 ).
Western Blot analysis of RNF144B expression in transfected 293T cell line (H00255488-T01) by RNF144B MaxPab polyclonal antibody.Lane 1: IBRDC2 transfected lysate(33.22 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RNF144B expression in transfected 293T cell line (H00255488-T02) by RNF144B MaxPab polyclonal antibody.Lane 1: RNF144B transfected lysate(33.60 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RNF144B expression in transfected 293T cell line (H00255488-T02) by RNF144B MaxPab polyclonal antibody.Lane 1: RNF144B transfected lysate(33.60 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of RNF144B transfected lysate using anti-RNF144B MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RNF144B MaxPab mouse polyclonal antibody (B01) (H00255488-B01).
Human ACHN; WB Suggested Anti-RNF144B Antibody Titration: 0.2-1 ug/ml. Positive Control: ACHN cell lysate; RNF144B antibody - middle region (ARP43479_P050) in Human ACHN cells using Western Blot
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to IBRDC2 on HeLa cell (antibody concentration 10 µg/ml).
RNF144B monoclonal antibody, clone 4F1 Western Blot analysis of IBRDC2 expression in IMR-32.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500696).
Anti-RNF144B mouse monoclonal antibody (TA500696) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500696),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500696), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody(TA500696),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunofluorescent staining of HepG2 cells using anti-RNF144B mouse monoclonal antibody (TA500698).
Anti-RNF144B mouse monoclonal antibody (TA500698) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500698), and then analyzed by flow cytometry.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500699).
Anti-RNF144B mouse monoclonal antibody (TA500699) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500699),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500699), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody(TA500699),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500704)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500704)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500704)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500704)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500704)
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500704).
Anti-RNF144B mouse monoclonal antibody (TA500704) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500704),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody(TA500704),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500704), and then analyzed by flow cytometry.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500705).
Anti-RNF144B mouse monoclonal antibody (TA500705) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500705),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500705), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500708).
Anti-RNF144B mouse monoclonal antibody (TA500708) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500708),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500708), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody(TA500708),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-RNF144B mouse monoclonal antibody (TA500709) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500709), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500709),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500710).
Anti-RNF144B mouse monoclonal antibody (TA500710) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500710),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500710), and then analyzed by flow cytometry.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500711).
Anti-RNF144B mouse monoclonal antibody (TA500711) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500711), and then analyzed by flow cytometry.
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500712), and then analyzed by flow cytometry.
Anti-RNF144B mouse monoclonal antibody (TA500722) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302 ).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RNF144B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500722),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody(TA500722),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500722), and then analyzed by flow cytometry.
Western blot analysis of extracts from HeLa cells, using RNF144B antibody.The lane on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA312549 | RNF144B antibody |
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| Rabbit polyclonal anti-RNF144B antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
$335.00
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| OriGene Technologies, Inc. | |||||||