RGS14 antibody
Principal name
RGS14 antibody
Alternative names for RGS14 antibody
Regulator of G-protein signaling 14
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of RGS14 antibody
- Kit Component:
- - KN204139G1, RGS14 gRNA vector 1 in pCas-Guide vector
- KN204139G2, RGS14 gRNA vector 2 in pCas-Guide vector
- KN204139D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Snow BE, Antonio L, Suggs S, Gutstein HB, Siderovski DP.
Molecular cloning and expression analysis of rat Rgs12 and Rgs14. Biochem Biophys Res Commun. 1997 Apr 28;233(3):770-7.
PMID: 9168931
RGS14 polyclonal antibody ( Cat # PAB6630 ) staining ( 0.5 µg/ml ) of Jurkat lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot analysis of RGS14 expression in transfected 293T cell line (H00010636-T01) by RGS14 MaxPab polyclonal antibody.Lane 1: RGS14 transfected lysate(61.40 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of RGS14 Antibody Cat.-No AP53650PU-N in U937 cell line lysates (35ug/lane). This demonstrates the RGS14 antibody detected the RGS14 protein (arrow).
Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N133/21 (left) or N52A/42 (right) TC supe
Immunoblot against lysates from wild-type (+/+) and RGS14 knockout (-/-) mouse brains probed with N133/21. Data courtesy of John Hepler (Emory).
Adult rat hippocampus immunohistochemistry
Adult rat brain membrane immunoblot
Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N133/21 (left) or N52A/42 (right) TC supe
Immunoblot against lysates from wild-type (+/+) and RGS14 knockout (-/-) mouse brains probed with N133/21. Data courtesy of John Hepler (Emory).
Adult rat hippocampus immunohistochemistry
Adult rat brain membrane immunoblot
TA302943 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
RGS14 antibody [C3], C-term detects RGS14 protein by Western blot analysis. A. 30 ug Jurkat whole lysate/extract. 7.5 % SDS-PAGE. RGS14 antibody [C3], C-term (TA308383) dilution: 1:500
Immunohistochemical analysis of paraffin-embedded GASTRIC CA, using RGS14(TA308383) antibody at 1:100 dilution.
Western blot analysis of extracts from NIH/3T3 cells, using RGS14 antibody.The lane on the right is treated with the synthesized peptide.
Host: Rabbit; Target Name: Rgs14; Sample Tissue: Mouse Stomach lysates; Antibody Dilution: 1.0 ug/ml
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| 73-170 | RGS14 antibody |
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![Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N133/21 (left) or N52A/42 (right) TC supe](https://m1.acris-antibodies.com/catalog/product/cache/2/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/NeuroMab/73-170-1.png) |
Mouse | IgG2a | N133/21 | Supernatant | Hu, Ms, Rt | C, ICC/IF, WB |
5 ml /
$490.00
|
| Antibodies Incorporated | |||||||
 +3 additional images |
Highly validated antibody!
|
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| 75-170 | RGS14 antibody |
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![Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N133/21 (left) or N52A/42 (right) TC supe](https://m1.acris-antibodies.com/catalog/product/cache/2/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/NeuroMab/75-170-1.png) |
Mouse | IgG2a | N133/21 | Purified | Hu, Ms, Rt | C, ICC/IF, WB |
0.1 ml /
$570.00
|
| Antibodies Incorporated | |||||||
| +3 additional images |
Highly validated antibody!
|
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