RFPL3 antibody
Principal name
RFPL3 antibody
Alternative names for RFPL3 antibody
Ret finger protein-like 3
SwissProt ID
O75679 (Human)
Gene ID
10738 (RFPL3)
Ncbi ID
9606, NP_001092005, NP_001092005.1, NM_006604, NP_006595, NM_001098535
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of RFPL3 antibody
- Kit Component:
- - KN206518G1, RFPL3 gRNA vector 1 in pCas-Guide vector
- KN206518G2, RFPL3 gRNA vector 2 in pCas-Guide vector
- KN206518D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Collins,J.E., Genome Biol. 5 (10), R84 (2004)
Western Blot analysis of RFPL3 expression in transfected 293T cell line (H00010738-T01) by RFPL3 MaxPab polyclonal antibody.Lane 1: RFPL3 transfected lysate(31.68 KDa).Lane 2: Non-transfected lysate.
Human Jurkat; WB Suggested Anti-RFPL3 Antibody Titration: 5.0ug/ml. Positive Control: Jurkat cell lysate; RFPL3 antibody - middle region (ARP43076_T100) in Human Jurkat cells using Western Blot
Human HepG2; WB Suggested Anti-RFPL3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; RFPL3 antibody - C-terminal region (ARP43077_P050) in Human HepG2 cells using Western Blot
Human COLO205; WB Suggested Anti-RFPL3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: COLO205 cell lysate; RFPL3 antibody - middle region (ARP57945_P050) in Human COLO205 cells using Western Blot
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RFPL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504731)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RFPL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504731)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RFPL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504731)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RFPL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504731)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RFPL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504731)
Anti-RFPL3 mouse monoclonal antibody (TA504731) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RFPL3(RC206518).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFPL3 (RC206518, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFPL3.
HEK293T cells transfected with either RC206518 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RFPL3 antibody(TA504731), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-RFPL3 antibody(TA504731),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-RFPL3 antibody(TA504731),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFPL3 (RC206518, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFPL3.
HEK293T cells transfected with either RC206518 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RFPL3 antibody(TA504732), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFPL3 (RC206518, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFPL3.
HEK293T cells transfected with either RC206518 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RFPL3 antibody(TA504733), and then analyzed by flow cytometry.
WB Suggested Anti-RFPL3 Antibody Titration: 5.0ug/ml; Positive Control: Jurkat cell lysate
WB Suggested Anti-RFPL3 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate
WB Suggested Anti-RFPL3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: COLO205 cell lysate
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA504731 | RFPL3 antibody |
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| RFPL3 mouse monoclonal antibody, clone OTI2G4 (formerly 2G4) | |||||||
 |
Mouse | IgG2b | OTI2G4 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
$379.00
|
| OriGene Technologies, Inc. | |||||||
 +9 additional images |
Highly validated antibody!
|
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| TA504732 | RFPL3 antibody |
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| RFPL3 mouse monoclonal antibody, clone OTI2B6 (formerly 2B6) | |||||||
 |
Mouse | IgG1 | OTI2B6 | Purified | Hu | F, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA504733 | RFPL3 antibody |
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| RFPL3 mouse monoclonal antibody, clone OTI2A6 (formerly 2A6) | |||||||
 |
Mouse | IgG1 | OTI2A6 | Purified | Hu | F, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306518 | RFPL3 (transcript variant 2) |
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| Recombinant protein of human ret finger protein-like 3 (RFPL3), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416536 | RFPL3 overexpression lysate |
|
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0.1 mg /
$295.00
|
|
| OriGene Technologies, Inc. |