RET antibody
Principal name
RET antibody
Alternative names for RET antibody
C-ret, CDHF12, Proto-oncogene tyrosine-protein kinase receptor ret, Cadherin family member 12
Ncbi ID
NP_065681.1, 9606, NP_066124, NP_065681, NP_000314.1, NP_066124.1, NM_001080780, NM_009050, NM_020630, NM_020975
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of RET antibody
The RET proto-oncogene on human chromosome 10q11.2, encodes a receptor tyrosine kise (RTK) activated by members of the glial cell line-derived neurotrophic factor (GDNF) ligand family (GDNF, neurturin, artemin, and persephin) in conjunction with a ligand-specific coreceptor (GFRa1-4). RET belongs to the cadherin superfamily, and it has been suggested that the ret gene is the result of a recombition between a gene encoding a tyrosine kise receptor and a gene encoding an ancestral cadherin at an early stage of evolution. The extracellular domain of RET comprises, as observed for other tyrosine kise receptors, a cysteine-rich domain, but also four cadherin-like domains. RET sigling is crucial for the development of the enteric nervous system. RET also regulates the development of sympathetic, parasympathetic, motor, and sensory neurons, and is necessary for the posttal maintence of dopaminergic neurons. Outside the nervous system, RET is crucial for development of the kidney and plays a key role in spermatogenesis. RET has attracted considerable clinical interest because of the range of mutations found in diverse conditions that include Hirschsprung disease and a variety of cancers involving the thyroid gland. RET-related cancers with thyroid involvement include sporadic and familial medullary thyroid carcinoma (MTC), multiple endocrine neoplasia 2 (MEN2) syndromes MEN2A and MEN2B, and papillary thyroid carcinoma (PTC).
Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and RET HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RET mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Detection limit for recombinant GST tagged RET is approximately 3ng/ml as a capture antibody.
Intracellular staining of SH-SY5Y cells with APC-conjugated anti-human Ret (filled histogram) or with isotype control antibody (open histogram).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with RET polyclonal antibody ( Cat # PAB3379 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of RET polyclonal antibody ( Cat # PAB3379 ) in SK-BR-3 cell lysate. RET ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of RET polyclonal antibody ( Cat # PAB3380 ) in SK-BR-3 cell lysate. RET ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with RET polyclonal antibody ( Cat # PAB3382 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
RET monoclonal antibody (M07), clone 1C6. Western Blot analysis of RET expression in NIH/3T3(Cat # L018V1 ).
RET monoclonal antibody (M07), clone 1C6 Western Blot analysis of RET expression in MCF-7 ( Cat # L046V1 ).
RET monoclonal antibody (M07), clone 1C6. Western Blot analysis of RET expression in PC-12(Cat # L012V1 ).
Detection limit for recombinant GST tagged RET is approximately 1ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Western blot analysis of anti-Ret Pab in SKBR3 cell lysate. Ret (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of Ret Antibody (C-term) in SKBR3 cell lysate. Ret (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of RET Antibody Cat.-No AP14410PU-N in MCF7 cell line lysates (35ug/lane). RET (arrow) was detected using the purified Pab.
Flow cytometric analysis of MCF-7 cells using RET Antibody (N-term) Cat.-No AP14410PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of RET (arrow) using rabbit polyclonal RET Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RET gene (Lane 2).
Western Blot analysis of RET expression in transfected 293T cell line (H00005979-T01) by RET MaxPab polyclonal antibody.Lane 1: RET transfected lysate(117.92 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between RET and MAPK1 HeLa cells were stained with anti-RET rabbit purified polyclonal 1:1200 and anti-MAPK1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
RET MaxPab rabbit polyclonal antibody. Western Blot analysis of RET expression in mouse liver.
RET MaxPab rabbit polyclonal antibody. Western Blot analysis of RET expression in human liver.
Western Blot analysis of RET expression in transfected 293T cell line (H00005979-T02) by RET MaxPab polyclonal antibody.Lane 1: RET transfected lysate(119.80 KDa).Lane 2: Non-transfected lysate.
Western blot analyzes of Ret antibody (Cat.-No.: AP20723PU-N) in extracts from K562 cells.
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis using RET monoclonal antibody against truncated RET-GST recombinant protein and RET(aa658-1063)/hIgGFc transfected CHO-K1 cell lysate.
Western blot analysis using RET antibody Cat.-No AM06194SU-N against truncated RET recombinant protein (Lane 1) and RET (aa 658-1063)-hIgGFc transfected CHO-K1 cell lysate (Lane 2).
Western blot analysis using RET antibody Cat.-No AM06202SU-N against truncated RET recombinant protein (Lane 1) and RET (aa658-1063)-hIgGFc transfected CHO-K1 cell lysate (Lane 2).
Immunofluorescence detection of human RET expressed in U2OS cells. RET was visualized using Chicken polyclonal antibody to human RET (dilution 1:500) and Goat anti-chicken IgY - H&L DyLight 550. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for RET specific signal and blue for nuclei.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (RC214268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.
Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805707)
Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805707)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (RC214268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (RC214268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.
Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805744)
Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805744)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (RC214268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.
Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805745)
Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805745)
Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805761)
Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805761)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (RC214268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.
Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805767)
Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805767)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (RC214268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.
Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805811)
Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805811)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (RC214268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.
Immunohistochemical staining of paraffin-embedded RET positive Human lung carcinoma tissue using anti-RET mouse monoclonal antibody. (TA805863; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded RET positive Human lung carcinoma tissue using anti-RET mouse monoclonal antibody. (TA805863; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (RC214268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.
Immunohistochemical staining of paraffin-embedded RET positive Human lung carcinoma tissue using anti-RET mouse monoclonal antibody. (TA805940; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded RET positive Human lung carcinoma tissue using anti-RET mouse monoclonal antibody. (TA805940; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (RC214268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.
Immunohistochemical staining of paraffin-embedded RET positive Human lung carcinoma tissue using anti-RET mouse monoclonal antibody. (TA805988; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded RET positive Human lung carcinoma tissue using anti-RET mouse monoclonal antibody. (TA805988; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (RC214268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET.
Immunohistochemical analysis of RET staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of RET staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of RET expression in K562 (A); HeLa (B) whole cell lysates.
Immunohistochemical analysis of RET staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of RET staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of RET expression in K562 (A) whole cell lysates.
Immunohistochemical analysis of RET (pY1062) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of RET (pY1062) staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of RET (pY1062) expression in K562 (A); mouse brain (B) whole cell lysates.
Western blot analysis of extracts from Jurkat cells, using Ret (Ab-905) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Ret (Ab-905) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HuvEc cells, using Ret (Ab-905) antibody.The picture on the right is treated with the synthesized peptide.
RET (Cat. #TA324337) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RET antibody detected the RET protein (arrow).
RET Monoclonal( Ascites) (Cat. #TA324337) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RET Monoclonal( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of RET Antibody (Ascites)(Cat#TA324337) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of Ret expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: 1,2. Anti-Ret (extracellular) antibody, (1:200). 3,4. Anti-Ret (extracellular) antibody, preincubated with the control peptide antigen.
Expression of Ret in rat DRG. Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-Ret (extracellular) antibody, (1:50). Ret staining (red) is detected in DRG neurons. Hoechst 33342 (blue) is used to stain cell nuclei.
Expression of Ret in human MCF-7 breast adenocarcinoma cells. Immunocytochemical staining of live intact human MCF-7 breast adenocarcinoma cells. A. Extracellular staining of cells with Anti-Ret (extracellular) antibody, (1:25), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of the two images.
Indirect flow cytometry analysis of live intact human THP-1 monocytic leukemia cell line: black line: Unstained cells + goat-anti-rabbit-AlexaFluor-647 secondary antibody. green line: Cells + Anti-Ret (extracellular) antibody, (1:20) + goat-anti-rabbit-AlexaFluor-647 secondary antibody.
Western blot analysis of Ret phosphorylation expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP14409PU-N | RET (C-term) antibody |
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Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||
| AP14410PU-N | RET (N-term) antibody |
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Rabbit | Ig | Purified | Hu | F, P, WB |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| AP14411PU-N | RET (C-term) antibody |
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Rabbit | Ig | Purified | Hu | P |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP20723PU-N | RET antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
$370.00
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP710121 | RET (C-term DDK tag, transcript variant 2) |
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| Recombinant protein of human ret proto-oncogene (RET), transcript variant 2, residues 708-1016aa, with C-terminal DDK tag,expressed in sf9 cells | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
$411.00
|
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| OriGene Technologies, Inc. | |||||
| TP710180 | RET (C-term DDK tag, transcript variant 2) |
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| Recombinant protein of human ret proto-oncogene (RET), transcript variant 2, residues 29-635aa, with C-terminal DDK tag, expressed in sf9, 20ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
$411.00
|
|
| OriGene Technologies, Inc. | |||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC214268L2V | Lenti ORF particles, RET (mGFP-tagged)-Human ret proto-oncogene (RET), transcript variant 2, 200 uL, >10^7 TU/mL |
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200 µl /
$2,370.00
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| OriGene Technologies, Inc. | ||
| RC214268L1V | Lenti ORF particles, RET (Myc-DDK-tagged)-Human ret proto-oncogene (RET), transcript variant 2, 200 uL, >10^7 TU/mL |
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200 µl /
$2,130.00
|
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| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211589L2V | Lenti ORF particles, Ret (GFP-tagged) - Mouse ret proto-oncogene (Ret), transcript variant 4, 200 uL, >10^7 TU/mL |
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200 µl /
$2,000.00
|
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| OriGene Technologies, Inc. | ||
| MR211589L1V | Lenti ORF particles, Ret (Myc-DDK-tagged) - Mouse ret proto-oncogene (Ret), transcript variant 4, 200 uL, >10^7 TU/mL |
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200 µl /
$1,790.00
|
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| OriGene Technologies, Inc. | ||