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PUS1 antibody

Principal name

PUS1 antibody

Alternative names for PUS1 antibody

PP8985, Pseudouridylate synthase 1, tRNA-uridine isomerase I, MLASA1, MGC11268, tRNA pseudouridine synthase A mitochondrial, tRNA pseudouridine(38-40) synthase, tRNA pseudouridylate synthase I

SwissProt ID

P07649 (Ecoli), Q4KM92 (Rat), Q9WU56 (Mouse), Q9Y606 (Human)

Gene ID

80324 (PUS1), 56361 (Pus1), 304567

Ncbi ID

9606, NP_001020733, NM_001025562, NP_001002019, NP_001002019.1, NP_079491, NP_001002020, NP_001020733.1, NM_019700, NM_001025561, NM_001025563

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)

Background of PUS1 antibody

Kit Component:: - KN314259G1, Pus1 gRNA vector 1 in pCas-Guide vector
- KN314259G2, Pus1 gRNA vector 2 in pCas-Guide vector
- KN314259D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

Bergmann, A.K., et al. Pediatr Blood Cancer 54(2):273-278 (2010).
Sibert, B.S., et al. RNA 14(9):1895-1906 (2008).
Rikova, K., et al. Cell 131(6):1190-1203 (2007).
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109 (2007).
Fernandez-Vizarra, E., et al. J. Med. Genet. 44(3):173-180 (2007).

Mouse SP2/0; WB Suggested Anti-PUS1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: SP2/0 cell lysate; PUS1 antibody - C-terminal region (ARP37261_P050) in Mouse SP2/0 cells using Western Blot

ARP37261_P050

Western Blot analysis of PUS1 expression in transfected 293T cell line (H00080324-T01) by PUS1 MaxPab polyclonal antibody.Lane 1: PUS1 transfected lysate(44 KDa).Lane 2: Non-transfected lysate.

H00080324-B01

Western Blot analysis of PUS1 expression in transfected 293T cell line (H00080324-T02) by PUS1 MaxPab polyclonal antibody.Lane 1: PUS1 transfected lysate(43.89 KDa).Lane 2: Non-transfected lysate.

H00080324-B02

Mouse Kidney; WB Suggested Anti-Pus1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Kidney; Pus1 antibody - N-terminal region (ARP37260_P050) in Mouse Kidney cells using Western Blot

ARP37260_P050

Formalin fixed, paraffin embedded human skeletal muscle stained with PUS1 Antibody (C-term) Cat.-No AP53521PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining

AP53521PU-N

Western blot analysis of PUS1 Antibody (C-term) Cat.-No AP53521PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the PUS1 antibody detected the PUS1 protein (arrow).