PTPRE antibody
Principal name
PTPRE antibody
Alternative names for PTPRE antibody
Protein-tyrosine phosphatase epsilon, R-PTP-epsilon, Receptor-type tyrosine-protein phosphatase epsilon
Ncbi ID
9606, NP_006495, ADZ16048.1, NP_006495.1, NM_130435, NP_569119, NP_035342.3, NM_011212, NM_053767, NM_006504
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)
Background of PTPRE antibody
PTPRE is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Two alternatively spliced transcript variants of this gene have been reported, one of which encodes a receptor-type PTP that possesses a short extracellular domain, a single transmembrane region, and two tandem intracytoplasmic catalytic domains; Another one encodes a PTP that contains a distinct hydrophilic N-terminus, and thus represents a nonreceptor-type isoform of this PTP. Studies of the similar gene in mice suggested the regulatory roles of this PTP in RAS related signal transduction pathways, cytokines induced SATA signaling, as well as the activation of voltage-gated K+ channels.The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Two alternatively spliced transcript variants of this gene have been reported, one of which encodes a receptor-type PTP that possesses a short extracellular domain, a single transmembrane region, and two tandem intracytoplasmic catalytic domains; Another one encodes a PTP that contains a distinct hydrophilic N-terminus, and thus represents a nonreceptor-type isoform of this PTP. Studies of the similar gene in mice suggested the regulatory roles of this PTP in RAS related signal transduction pathways, cytokines induced SATA signaling, as well as the activation of voltage-gated K+ channels.
General readings
Weiss,B. (2008) J. Biol. Chem. 283 (8), 4612-4621
Detection limit for recombinant GST tagged PTPRE is approximately 10ng/ml as a capture antibody.
PTPRE polyclonal antibody (A01), Lot # 051017JC01 Western Blot analysis of PTPRE expression in Hela NE ( Cat # L013V3 ).
Human 293T; WB Suggested Anti-PTPRE Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate; PTPRE antibody - middle region (ARP46732_P050) in Human 293T cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRE (RC207950, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRE.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500994, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500994, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500994, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500994, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500994, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500994, Dilution 1:50)
Anti-PTPRE mouse monoclonal antibody (TA500994) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE(RC207950).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRE (RC207950, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRE.
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500995, Dilution 1:50)
Anti-PTPRE mouse monoclonal antibody (TA500995) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE(RC207950).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRE (RC207950, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRE.
Immunofluorescent staining of HT29 cells using anti-PTPRE mouse monoclonal antibody (TA501025).
Anti-PTPRE mouse monoclonal antibody (TA501025) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE(RC207950).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRE (RC207950, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRE.
HEK293T cells transfected with either pCMV6-ENTRY PTPRE(RC207950)(Red) or empty vector control plasmid(Blue) were immunostained with anti-PTPRE mouse monoclonal(TA501025), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501026, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501026, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501026, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501026, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501026, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501026, Dilution 1:50)
Anti-PTPRE mouse monoclonal antibody (TA501026) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE(RC207950).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRE (RC207950, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRE.
Anti-PTPRE mouse monoclonal antibody (TA501027) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE(RC207950 ).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRE (RC207950, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRE.
HEK293T cells transfected with either RC207950 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PTPRE antibody(TA501027), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTPRE mouse monoclonal antibody. (TA501028; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (TA501028; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PTPRE mouse monoclonal antibody. (TA501028; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (TA501028; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (TA501028; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PTPRE mouse monoclonal antibody. (TA501028; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Anti-PTPRE mouse monoclonal antibody (TA501028) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE(RC207950).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRE (RC207950, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRE.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-PTPRE monoclonal antibody at 1:200 dilution.
Flow cytometric Analysis of Malme-3M cells, using anti-PTPRE antibody(TA501028),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501029, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501029, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501029, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501029, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501029, Dilution 1:50)
Anti-PTPRE mouse monoclonal antibody (TA501029) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE(RC207950).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRE (RC207950, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRE.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (TA501051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunofluorescent staining of HT29 cells using anti-PTPRE mouse monoclonal antibody (TA501051).
Anti-PTPRE mouse monoclonal antibody (TA501051) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE(RC207950).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRE (RC207950, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRE.
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-PTPRE monoclonal antibody at 1:200 dilution.
Flow cytometric Analysis of Malme-3M cells, using anti-PTPRE antibody(TA501051),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-PTPRE mouse monoclonal antibody (TA501073) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE(RC207950 ).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRE (RC207950, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRE.
HEK293T cells transfected with either RC207950 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PTPRE antibody(TA501073), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-PTPRE antibody(TA501073),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-PTPRE antibody(TA501073),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501230, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501230, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501230, Dilution 1:50)
Anti-PTPRE mouse monoclonal antibody (TA501230) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE(RC207950 ).
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-PTPRE monoclonal antibody at 1:200.
HEK293T cells transfected with either RC207950 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PTPRE antibody(TA501230), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PTPRE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501233, Dilution 1:50)
Anti-PTPRE mouse monoclonal antibody (TA501233) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE(RC207950 ).
HEK293T cells transfected with either RC207950 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PTPRE antibody(TA501233), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PTPRE mouse monoclonal antibody. (TA501234; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Anti-PTPRE mouse monoclonal antibody (TA501234) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE(RC207950 ).
Flow cytometric Analysis of Malm3-3m cells, using anti-PTPRE antibody(TA501234),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC207950 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PTPRE antibody(TA501234), and then analyzed by flow cytometry.
Predicted cell location: Cytoplasm. Positive control: Human ovarian and cervical cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using PTPRE antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human ovarian and cervical cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using PTPRE antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human breast and lung cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using PTPRE antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human breast and lung cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using PTPRE antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x200)
Anti-PTPRE antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3-6 ug/ml.
Anti-PTPRE antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3-6 ug/ml.
Anti-PTPRE / PTP Epsilon antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PTPRE / PTP Epsilon antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PTPRE / PTP Epsilon antibody IHC of human Nerves in Prostate Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PTPRE / PTP Epsilon antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PTPRE antibody IHC of human colon, submucosal vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PTPRE / PTP Epsilon antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PTPRE antibody IHC of human colon, submucosal vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PTPRE antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3-32 ug/ml.
Anti-PTPRE antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3-32 ug/ml.
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307950 | PTPRE (transcript variant 1) |
||||
| Recombinant protein of human protein tyrosine phosphatase, receptor type, E (PTPRE), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP317899 | PTPRE (transcript variant 2) |
||||
| Recombinant protein of human protein tyrosine phosphatase, receptor type, E (PTPRE), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||