PSMB7 antibody
Principal name
PSMB7 antibody
Alternative names for PSMB7 antibody
Proteasome subunit beta type-7, Proteasome 20S beta 7, Macropain chain Z, Proteasome subunit Z, Multicatalytic endopeptidase complex chain Z
SwissProt ID
A1XQU1 (Pig), P25043 (Yeast), P70195 (Mouse), Q09841 (Schpo), Q2TBP0 (Bovin), Q99436 (Human), Q9JHW0 (Rat)
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Paraffin Sections (P)
Background of PSMB7 antibody
The proteasome is a multicatalytic proteise complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit). [provided by RefSeq].
General readings
Wang X., et al. (2007) Biochemistry. 46:3553-3565Rho J.H., et al. (2008) J. Proteome Res. 7:2959-2972
Western Blot analysis of PSMB7 expression in transfected 293T cell line (H00005695-T02) by PSMB7 MaxPab polyclonal antibody.Lane 1: PSMB7 transfected lysate(30.58 KDa).Lane 2: Non-transfected lysate.
AP21518PU-N PSMB7 antibody staining of HEK293 lysate at 0.3 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504368)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504368)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504368)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504368)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504368)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504368)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504368)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504368)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504368)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504368)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504368)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504368)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMB7 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB7 (RC201799, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB7.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB7 (RC201799, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB7.
Anti-PSMB7 mouse monoclonal antibody (TA504383) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMB7(RC201799).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB7 (RC201799, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB7.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMB7 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504429)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504429)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504429)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504429)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504429)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504429)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504429)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504429)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504429)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504429)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504429)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB7 (RC201799, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB7.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMB7 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB7 (RC201799, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB7.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMB7 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB7 (RC201799, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB7.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMB7 monoclonal antibody.
Anti-PSMB7 mouse monoclonal antibody (TA504638) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMB7(RC201799).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB7 (RC201799, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB7.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMB7 monoclonal antibody.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP55209SU-N | PSMB7 pSer214 antibody |
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Rabbit | Serum | Hu | C, E, WB |
0.2 ml /
$430.00
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| OriGene Technologies GmbH | |||||||