PRMT7 antibody
Principal name
PRMT7 antibody
Alternative names for PRMT7 antibody
KIAA1933, Protein arginine N-methyltransferase 7, Histone-arginine N-methyltransferase PRMT7
Ncbi ID
9606, NP_001171753.1, NP_061896, NM_145404, NP_663379.1, NM_001184824
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of PRMT7 antibody
- Kit Component:
- - KN313939G1, Prmt7 gRNA vector 1 in pCas-Guide vector
- KN313939G2, Prmt7 gRNA vector 2 in pCas-Guide vector
- KN313939D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Immunofluorescence of purified MaxPab antibody to PRMT7 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of PRMT7 expression in transfected 293T cell line (H00054496-T01) by PRMT7 MaxPab polyclonal antibody.Lane 1: PRMT7 transfected lysate(76.12 KDa).Lane 2: Non-transfected lysate.
Figure 2. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Figure 1. Western blot analysis of PRMT7 (arrow) using rabbit polyclonal PRMT7 Antibody (N-term) (Cat.#AP11015PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT7 gene (Lane 2) (Origene Technologies).
Kidney: Foramlin-Fixed Paraffin-Embedded (FFPE)
Immunofluorescence of purified MaxPab antibody to PRMT7 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of PRMT7 expression in transfected 293T cell line (H00054496-T01) by PRMT7 MaxPab polyclonal antibody.Lane 1: PRMT7 transfected lysate(76.12 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PRMT7 expression in transfected 293T cell line (H00054496-T01) by PRMT7 MaxPab polyclonal antibody.Lane 1: PRMT7 transfected lysate(78.50 KDa).Lane 2: Non-transfected lysate.
Human 293T; Host:Rabbit. Target Name:PRMT7. Sample Tissue:Human 293T. Antibody Dilution: 1.0ug/ml.; PRMT7 antibody - N-terminal region (ARP40191_P050) in Human 293T cells using Western Blot
Hum. Adult Placenta; Host:Rabbit. Target Name:PRMT7. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; PRMT7 antibody - N-terminal region (ARP40191_P050) in Hum. Adult Placenta cells using Western Blot
Hum. Fetal Muscle; Host:Rabbit. Target Name:PRMT7. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; PRMT7 antibody - N-terminal region (ARP40191_P050) in Hum. Fetal Muscle cells using Western Blot
Human Liver; WB Suggested Anti-PRMT7 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Liver; PRMT7 antibody - N-terminal region (ARP40191_P050) in Human Liver cells using Western Blot
Mouse Thymus; WB Suggested Anti-Prmt7 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Thymus; Prmt7 antibody - C-terminal region (ARP40192_P050) in Mouse Thymus cells using Western Blot
(0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of PRMT7 using Jurkat cell lysate (Lane1 & 2)
Predicted band size: 78 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes)
Predicted band size: 78 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds)
Western Blot: PRMT7 Antibody [TA336349] - Analysis of PRMT7 in (A) HeLa and (B) NIH 3T3 cell lysate using PRMT7 antibody at 2 ug/ml.
WB Suggested Anti-PRMT7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Liver
Host: Rabbit; Target Name: PRMT7; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: PRMT7; Sample Tissue: Human 293T; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: PRMT7; Sample Tissue: Human Fetal Muscle; Antibody Dilution: 1.0 ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP08401PU-N | PRMT7 (346-360) antibody |
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Rabbit | IgG | Aff - Purified | Hu, Ms | P, WB |
50 µg /
$425.00
|
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| OriGene Technologies GmbH | |||||||
| AP11015PU-N | PRMT7 (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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