PRMT3 antibody
Principal name
PRMT3 antibody
Alternative names for PRMT3 antibody
HRMT1L3, Protein arginine N-methyltransferase 3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein
Ncbi ID
NP_005779, 9606, ACE87112.1, NM_005788, NP_005779.1, NP_598501.1, BC050775, NM_133740, NM_053557, NM_001145166
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of PRMT3 antibody
- Kit Component:
- - KN313936G1, Prmt3 gRNA vector 1 in pCas-Guide vector
- KN313936G2, Prmt3 gRNA vector 2 in pCas-Guide vector
- KN313936D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Tang J, Gary JD, et al. (1998). J Biol Chem. 273(27):16935-45.Choi S, Jung CR, et al. (2008). Biochim Biophys Acta. 1780(9):1062-9.
Human HepG2; WB Suggested Anti-PRMT3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; PRMT3 antibody - middle region (ARP32141_P050) in Human HepG2 cells using Western Blot
HRMT1L3 polyclonal antibody (A01), Lot # 061025JCS1 Western Blot analysis of HRMT1L3 expression in 293 ( Cat # L026V1 ).
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PRMT3 polyclonal antibody ( Cat # PAB2171 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of PRMT3 polyclonal antibody ( Cat # PAB2171 ) in whole HL-60 cell lysate : PRMT3 ( Arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of PRMT3 polyclonal antibody ( Cat # PAB2172 ) in whole HL-60 cell lysate : PRMT3 ( Arrow ) was detected using purified polyclonal antibody ( Lane B ) but not pre-immune serum ( lane A ) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Figure 2. Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 antibody (N-term) (Cat. #AP11007PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Figure 1. Western blot analysis of anti-PRMT3 Pab (Cat. #AP11007PU-N)in whole HL60 cell lysate: PRMT3 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Figure 3. Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 Antibody (C-term) (Cat.#AP11008PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Figure 2. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Figure 1. Western blot analysis of anti-PRMT3 Pab (Cat. #AP11008PU-N)in whole HL60 cell lysate: PRMT3 (Arrow) was detected using purified Pab (Lane B). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
PRMT3 monoclonal antibody (M16), clone 3G4. Western Blot analysis of PRMT3 expression in HepG2(Cat # L019V1 ).
Human Muscle; WB Suggested Anti-PRMT3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; PRMT3 antibody - middle region (ARP40183_P050) in Human Muscle cells using Western Blot
HCT116; Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.; PRMT3 antibody - middle region (ARP40183_P050) in HCT116 using CHIP
(1ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon and cervical cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PRMT3 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon and cervical cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using PRMT3 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)
WB Suggested Anti-PRMT3 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate
WB Suggested Anti-PRMT3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Muscle
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA311343 | PRMT3 (468-421) antibody |
||||||
| Goat Anti-PRMT3 (aa468-421) Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
$360.00
|
||
| OriGene Technologies, Inc. | |||||||