PRKACA antibody
Principal name
PRKACA antibody
Alternative names for PRKACA antibody
PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha
Ncbi ID
NP_002721, NP_002721.1, 9606, 9913, NM_002730., NP_997401, NM_008854, NM_002730
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot)
Background of PRKACA antibody
Protein kinase A (PKA) also known as cAMP-dependent protein kinase, is a 40 kDa protein kinase that phosphorylates serine or threonine residues in target proteins in response to elevated levels of cAMP. The second messenger cAMP is produced in response to a wide variety of hormones & neurotransmitters and regulates cellular processes such as cell growth and differentiation, ion channel conductivity, synaptic release of neurotransmitters & gene transcription. The principle intracellular target for cAMP is PKA. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits. Two major PKA isozymes, type I (PKAI) and type II (PKAII) consist of RI and RII respectively, complexed with C. Three different C subunits (alpha, beta, and gamma) and four different R subunits (RI-alpha, RI-beta, RII-alpha, and RII-beta) have been identified. It has been suggested that the complex structure of PKA may be necessary for it to accomplish such diverse functions.
Western blot analysis of PKA expression in lysates from Jurkat cells (Lane 1 & 2), mouse small intestine (Lane 3) and rat kidney (Lane 4).
Dot Blot analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (SM3155P). The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot. Lane 1: GST-Akt1Lane 2: GST-Akt2Lane 3: GST-Akt3Lane 4: GST-PKAc alphaLane 5: GST-PKAc betaLane 6: GST-PKAc gammaLane 7: GST-MEK 1Lane 8: GST
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKACA polyclonal antibody ( Cat # PAB4529 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
The PRKACA polyclonal antibody ( Cat # PAB4529 ) is used in Western blot to detect PRKACA alpha in mouse heart tissue lysate .
The PRKACA polyclonal antibody ( Cat # PAB4529 ) is used in Western blot to detect PRKACA alpha in Jurkat cell lysate .
Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K3 and PRKACA HeLa cells were stained with anti-MAP3K3 rabbit purified polyclonal 1:1200 and anti-PRKACA mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Detection limit for recombinant GST tagged PRKACA is approximately 0.1ng/ml as a capture antibody.
Western blot (WB) analysis of PKAalpha/beta cat antibody (Cat.-No.: AP01230PU-N) in extracts from COLO cells.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
The anti-PKA/C alpha/beta Pab is used in Western blot to detect PKA/C alpha in Jurkat cell lysate (lane 1) and mouse heart tissue lysate (lane 2).
Western Blot analysis of PRKACA expression in transfected 293T cell line (H00005566-T02) by PRKACA MaxPab polyclonal antibody.Lane 1: PRKACA transfected lysate(40.60 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of PRKACA expression in lysates from Jurkat cells ( Lane 1 ) , mouse small intestine ( Lane 2 ) and rat kidney ( Lane 3 ) .
Dot Blot analysis of GST and GST-fusion proteins using PRKACA monoclonal antibody, clone 6D2.1 ( Cat # MAB3591 ) and GST monoclonal antibody, clone S-tag-05 ( Cat # MAB3651 ).The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.Lane 1 : GST-Akt1.Lane 2 : GST-Akt2.Lane 3 : GST-Akt3.Lane 4 : GST-PKAc alpha.Lane 5 : GST-PKAc beta.Lane 6 : GST-PKAc gamma.Lane 7 : GST-MEK 1.Lane 8 : GST.
Western blot analysis of PRKACA Antibody (N-term K82) (AP17667PU-N) in NCI-H460 cell line lysates (35ug/lane). PRKACA (arrow) was detected using the purified Pab.
Human Placenta; WB Suggested Anti-PRKACA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Placenta; PRKACA antibody - N-terminal region (ARP57827_P050) in Human Placenta cells using Western Blot
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA α/β CAT (Phospho-Thr197) antibody AP55702PU-N (left)or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from mouse brain cells using PKA α/β CAT (Phospho-Thr197) Antibody AP55702PU-N.The lane on the right is treated with the antigen-specific peptide.
Immunofluorescence staining of methanol-fixed A549 cells using PKA α/β CAT (Phospho-Thr197) Antibody AP55702PU-N.
Immunohistochemical analysis of PKA C alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of PKA C alpha/beta staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of PKA C alpha/beta expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.
Immunohistochemical analysis of PKA C alpha/beta/gamma staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of PKA C alpha/beta/gamma staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of PKA C alpha/beta/gamma expression in HEK293T (A); mouse liver (B); rat liver (C) whole cell lysates.
Sample (30 ug of whole cell lysate). A: NIH-3T3. B: C2C12. 10% SDS PAGE. TA308159 diluted at 1:1000.
Sample(30 ug of whole cell lysate). A:HeLa S3. 12% SDS PAGE. TA308159 diluted at 1:500
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PKA alpha(TA308159) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
Western blot analysis of extracts from mouse brain cells, using PKA CAT (Ab-197) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PKA CAT (Ab-197) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using PKA CAT (Ab-197) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from Jurkat cells and COLO cells, using KAPC A/B antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-PRKACA Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Muscle
WB Suggested Anti-PRKACA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Placenta
Western blot analysis on Jurkat and K562 cell lysate using KAPC A/B Antibody
Western blot analysis of extracts from mouse brain cells, using PKA a/ß CAT (Phospho-Thr197) antibodyThe lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PKA a/ß CAT (Phospho-Thr197) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using PKA a/ß CAT (Phospho-Thr197) antibody.The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP55702PU-N | PRKACA / PRKACB pThr197 antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
$300.00
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| OriGene Technologies GmbH | |||||||
 +2 additional images |
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| AP55702PU-S | PRKACA / PRKACB pThr197 antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
50 µg /
$230.00
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| OriGene Technologies GmbH | |||||||
| +2 additional images |
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