PPP1R14D antibody
Principal name
PPP1R14D antibody
Alternative names for PPP1R14D antibody
GBPI, GBPI-1, Protein phosphatase 1 regulatory subunit 14D, Gastrointestinal and brain-specific PP1-inhibitory protein 1
Ncbi ID
NP_060196.1, NM_017726, NP_060196, NP_082380.1, NM_028104, NM_172011, NM_001130143
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)
Background of PPP1R14D antibody
- Kit Component:
- - KN313735G1, Ppp1r14d gRNA vector 1 in pCas-Guide vector
- KN313735G2, Ppp1r14d gRNA vector 2 in pCas-Guide vector
- KN313735D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunohistochemical analysis of PPP1R14D staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PPP1R14D expression in Jurkat (A); HepG2 (B) whole cell lysates.
Western blot analysis of extracts from COLO cells, HepG2 cells and HeLa cells, using PPP1R14D antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-PPP1R14D Antibody; Titration: 1.0 ug/ml; Positive Control: Jurkat Whole Cell
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP311763 | PPP1R14D (transcript variant 1) |
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| Recombinant protein of human protein phosphatase 1, regulatory (inhibitor) subunit 14D (PPP1R14D), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
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| OriGene Technologies, Inc. | |||||