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POLR2G antibody

Principal name

POLR2G antibody

Alternative names for POLR2G antibody

RNA polymerase II subunit B7, RPB7, RPB19, DNA-directed RNA polymerase II subunit RPB7, DNA-directed RNA polymerase II subunit G, RNA polymerase II 19 kDa subunit

SwissProt ID

O14459 (Schpo), P34087 (Yeast), P38421 (Arath), P62487 (Human), P62488 (Mouse), P62489 (Rat), Q5E9B8 (Bovin), Q7ZW41 (Danre)

Gene ID

5436 (POLR2G), 117017 (Polr2g), 67710

Ncbi ID

9606, NP_002687, NP_002687.1, NM_053948, NM_002696, NP_080605.1

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Sh (Sheep)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P)

Background of POLR2G antibody

Kit Component:: - KN313603G1, Polr2g gRNA vector 1 in pCas-Guide vector
- KN313603G2, Polr2g gRNA vector 2 in pCas-Guide vector
- KN313603D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

Cojocaru, M., et al. Biochem. J. 409(1):139-147(2008)
Ujvari, A., et al. Nat. Struct. Mol. Biol. 13(1):49-54(2006)
Meka, H., et al. Nucleic Acids Res. 33(19):6435-6444(2005)
Lehner, B., et al. Genomics 83(1):153-167(2004)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671(2003)

Western Blot analysis of POLR2G expression in transfected 293T cell line (H00005436-T01) by POLR2G MaxPab polyclonal antibody.Lane 1: POLR2G transfected lysate(18.92 KDa).Lane 2: Non-transfected lysate.

H00005436-B01

Western blot analysis of POLR2G Antibody (C-term) Cat.-No AP53385PU-N in Jurkat cell line lysates (35ug/lane). This demonstrates the POLR2G antibody detected the POLR2G protein (arrow).

AP53385PU-N

Flow cytometric analysis of Jurkat cells using POLR2G Antibody (C-term) Cat.-No AP53385PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

AP53385PU-N

Approx 21kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 19.3kDa according to NP_002687.1). Recommended concentration: 1-3ug/ml.