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POFUT2 antibody

Principal name

POFUT2 antibody

Alternative names for POFUT2 antibody

C21orf80, FUT13, KIAA0958, O-FucT-2, GDP-fucose protein O-fucosyltransferase 2, Peptide-O-fucosyltransferase 2

SwissProt ID

Q8VHI3 (Mouse), Q8WR51 (Caeel), Q9W589 (Drome), Q9Y2G5 (Human), Q9Y2G5-1

Gene ID

23275 (POFUT2), 80294 (Pofut2)

Ncbi ID

NP_056042, NP_598368, NP_056042.1, 9606, NM_133635, NM_015227, NM_030262, NP_084538.2

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of POFUT2 antibody

Fucose is typically found as a termil modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008]. Transcript Variant: This variant (3) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (C) is longer and differs in the C-terminus compared to isoform A. ##Evidence-Data-START## Transcript exon combition :: AY066015.2, BC064623.1 [ECO:0000332] Rseq introns :: single sample supports all introns ERS025095 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

General readings

Feng, T., et al. Hum. Genet. 128(3):269-280(2010)
Luo, Y., et al. J. Biol. Chem. 281(14):9385-9392(2006)
Luo, Y., et al. J. Biol. Chem. 281(14):9393-9399(2006)
Menzel, O., et al. Genomics 84(2):320-330(2004)
Martinez-Duncker, I., et al. Glycobiology 13 (12), 1C-5C (2003)

Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with POFUT2 Antibody (Center) Cat.-No AP53376PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of POFUT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

AP53376PU-N

Western blot analysis of POFUT2 Antibody (Center) Cat.-No AP53376PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the POFUT2 antibody detected the POFUT2 protein (arrow).

AP53376PU-N

Human Stomach; POFUT2 antibody - C-terminal region (ARP45854_T100) in Human Stomach cells using Immunohistochemistry

ARP45854_T100

Human HepG2; WB Suggested Antibody Titration: 2.5 ug/ml. Positive Control: HepG2. There is BioGPS gene expression data showing that POFUT2 is expressed in HepG2.; POFUT2 antibody - C-terminal region (ARP45854_T100) in Human HepG2 cells using Western Blot

ARP45854_T100

Transfected 293T; WB Suggested Anti-POFUT2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; POFUT2 antibody - N-terminal region (ARP45855_P050) in Transfected 293T cells using Western Blot

ARP45855_P050

Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using POFUT2 Antibody. The picture on the right is treated with the synthesized peptide.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POFUT2 (RC209505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POFUT2.

TA504972

HEK293T cells transfected with either RC209505 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POFUT2 antibody(TA504972), and then analyzed by flow cytometry.

TA504972

TA504982

HEK293T cells transfected with either RC209505 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POFUT2 antibody(TA504982), and then analyzed by flow cytometry.

TA504982

TA504983

TA505004

HEK293T cells transfected with either RC209505 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POFUT2 antibody(TA505004), and then analyzed by flow cytometry.