PNPO antibody
Principal name
PNPO antibody
Alternative names for PNPO antibody
Pyridoxine-5'-phosphate oxidase, Pyridoxamine-phosphate oxidase
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of PNPO antibody
- Kit Component:
- - KN200133G1, PNPO gRNA vector 1 in pCas-Guide vector
- KN200133G2, PNPO gRNA vector 2 in pCas-Guide vector
- KN200133D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Musayev, F.N., et al. J. Biol. Chem. 284(45):30949-30956(2009)
Khayat, M., et al. Mol. Genet. Metab. 94(4):431-434(2008)
Song, H., et al. Schizophr. Res. 97 (1-3), 264-270 (2007)
Mills, P.B., et al. Hum. Mol. Genet. 14(8):1077-1086(2005)
Kang, J.H., et al. Eur. J. Biochem. 271(12):2452-2461(2004)
Musayev, F.N., et al. Protein Sci. 12(7):1455-1463(2003)
PNPO polyclonal antibody (A01), Lot # 060529JCS1 Western Blot analysis of PNPO expression in MES-SA/Dx5 ( Cat # L021V1 ).
Western Blot analysis of PNPO expression in transfected 293T cell line (H00055163-T01) by PNPO MaxPab polyclonal antibody.Lane 1: PNPO transfected lysate(28.71 KDa).Lane 2: Non-transfected lysate.
Human Muscle; WB Suggested Anti-PNPO Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; PNPO antibody - N-terminal region (ARP58515_P050) in Human Muscle cells using Western Blot
Recombinant human PNPO, 57-261aa, His-tagged
The extracts of HepG2(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNPO (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis:The extracts of HepG2 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNPO (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis of PNPO Antibody (C-term) Cat.-No AP53372PU-N in 293 cell line lysates (35ug/lane). PNPO (arrow) was detected using the purified Pab.
Anti-PNPO mouse monoclonal antibody (TA503178) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PNPO(RC200133).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO (RC200133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNPO.
HEK293T cells transfected with either RC200133 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PNPO antibody(TA503178), and then analyzed by flow cytometry.
Anti-PNPO mouse monoclonal antibody (TA503249) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PNPO(RC200133).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO (RC200133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNPO.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PNPO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503356)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503356)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503356)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503356)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO (RC200133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNPO.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503506)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO (RC200133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNPO.
Flow cytometric Analysis of Jurkat cells, using anti-PNPO antibody(TA503506),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-PNPO antibody(TA503506),(Red), compared to a nonspecific negative control antibody,(Blue).
WB Suggested Anti-PNPO Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503506 | PNPO antibody |
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| PNPO mouse monoclonal antibody, clone OTI1G9 (formerly 1G9) | |||||||
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Mouse | IgG2a | OTI1G9 | Purified | Hu | F, P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
 +14 additional images |
Highly validated antibody!
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| TA503178 | PNPO antibody |
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| PNPO mouse monoclonal antibody, clone OTI2F8 (formerly 2F8) | |||||||
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Mouse | IgG1 | OTI2F8 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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