PIWIL4 antibody
Principal name
PIWIL4 antibody
Alternative names for PIWIL4 antibody
Piwi-like protein 4, HIWI2, PIWI, MIWI2
Ncbi ID
9606, NP_689644, NM_152431, ABM85351.1, NP_689644.2, NM_177905, NP_808573.2
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of PIWIL4 antibody
- Kit Component:
- - KN313350G1, Piwil4 gRNA vector 1 in pCas-Guide vector
- KN313350G2, Piwil4 gRNA vector 2 in pCas-Guide vector
- KN313350D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
"The induction of H3K9 methylation by PIWIL4 at the p16Ink4a locus." Sugimoto K., Kage H., Aki N., Sano A., Kitagawa H., Nagase T., Yatomi Y., Ohishi N., Takai D. Biochem. Biophys. Res. Commun. 359:497-502 (2007) [PubMed: 17544373]
Western Blot analysis of PIWIL4 expression in transfected 293T cell line (H00143689-T01) by PIWIL4 MaxPab polyclonal antibody.Lane 1: PIWIL4 transfected lysate(93.50 KDa).Lane 2: Non-transfected lysate.
PIWIL4 MaxPab rabbit polyclonal antibody. Western Blot analysis of PIWIL4 expression in Jurkat.
Human Brain; WB Suggested Anti-PIWIL4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain; PIWIL4 antibody - N-terminal region (ARP52960_P050) in Human Brain cells using Western Blot
Human HT1080; WB Suggested Anti-PIWIL4 Antibody Titration: 0.2-1 ug/ml. Positive Control: HT1080 cell lysate; PIWIL4 antibody - N-terminal region (ARP52961_P050) in Human HT1080 cells using Western Blot
Predicted band size: 97 kDa . Positive control: Hela, 231 and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Hela cell lysate Lane2: 231 cell lysate Lane3: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PIWIL4 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using PIWIL4 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
WB Suggested Anti-PIWIL4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human brain
WB Suggested Anti-PIWIL4 Antibody Titration: 0.2-1 ug/ml; Positive Control: HT1080 cell lysate
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306117 | PIWIL4 |
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| Recombinant protein of human piwi-like 4 (Drosophila) (PIWIL4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
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| OriGene Technologies, Inc. | |||||