PIM1 antibody
Principal name
PIM1 antibody
Alternative names for PIM1 antibody
Pim-1, Proto-oncogene serine/threonine-protein kinase pim-1
SwissProt ID
P06803 (Mouse), P11309 (Human), P26794 (Rat), Q95LJ0 (Felca), Q9N0P9 (Bovin), Q9YHZ5 (Danre)
Gene ID
5292 (PIM1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))
Background of PIM1 antibody
The protein encoded by this gene belongs to the Ser/Thr protein kise family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic maligncies and in prostate cancer. It plays a role in sigl transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of altertive in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011].
General readings
Koike N., et al. (2000) FEBS Lett. 467:17-21Ionov Y., et al. (2003) Anticancer Res. 23:167-178
Detection limit for recombinant GST tagged PIM1 is approximately 0.1ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to PIM1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged PIM1 is approximately 0.1ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to PIM1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 1.5 ug/ml]
Immunoperoxidase of monoclonal antibody to PIM1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 1 ug/ml]
Detection limit for recombinant GST tagged PIM1 is approximately 0.3ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the PIM1 polyclonal antibody ( Cat # PAB2304 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of PIM1 polyclonal antibody ( Cat # PAB2304 ) in A-549 cell lysate. PIM1 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot (WB) analysis of Pim-1 antibody (Cat.-No.: AP01350PU-N) in extracts from Jurkat cells.
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PIM1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of PIM1 (arrow) using PIM1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIM1 gene (Lane 2) (Origene Technologies).
Hum. Fetal Lung; Host: Rabbit . Target Name: PIM1 . Sample Tissue: Human Fetal Lung . Antibody Dilution: 1.0ug/ml.; PIM1 antibody - N-terminal region (ARP56389_P050) in Hum. Fetal Lung cells using Western Blot
Human Heart; WB Suggested Anti-PIM1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human heart; PIM1 antibody - N-terminal region (ARP56389_P050) in Human Heart cells using Western Blot
Human 293T; WB Suggested Anti-PIM1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. PIM1 is supported by BioGPS gene expression data to be expressed in HEK293T.; PIM1 antibody - N-terminal region (ARP56390_P050) in Human 293T cells using Western Blot
AP23807PU PIM1 antibody staining of Human Bone Marrow.
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to PIM1 on HeLa cell . [antibody concentration 10 µg/ml]
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to PIM1 on HeLa cell . [antibody concentration 10 µg/ml]
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to PIM1 on HeLa cell . [antibody concentration 10 µg/ml]
Western blot analysis of extracts from HUVEC cells treated with PMA using Pim-1 (Phospho-Tyr309) Antibody AP55710PU-N.The lane on the right is treated with the antigen-specific peptide.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
(LEFT)Western blot analysis of anti-PIM1 Pab in A549 cell lysate. PIM1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of PIM1 (arrow) using PIM1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIM1 gene (Lane 2) (Origene Technologies).
Anti-PIM1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Western blot analysis of PIM1 using Jurkat cell lysate.
WB Suggested Anti-PIM1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human heart
Host: Rabbit; Target Name: PIM1; Sample Tissue: Human Fetal Lung; Antibody Dilution: 1.0ug/ml
WB Suggested Anti-PIM1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysatePIM1 is supported by BioGPS gene expression data to be expressed in HEK293T
Western blot analysis on HuvEc cell lysate using Pim-1 Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM31122PU-N | PIM1 antibody |
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 |
Mouse | IgG1 | 6A2 | Purified | Hu | E, ICC/IF, P |
50 µg /
$475.00
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||
| AM31123PU-N | PIM1 antibody |
||||||
 |
Mouse | IgG2a | 3F5 | Purified | Hu | E, ICC/IF, P |
50 µg /
$475.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AM31764PU-N | PIM1 antibody |
||||||
 |
Mouse | IgG2a | X1 | Purified | Hu | E, ICC/IF, P |
50 µg /
$475.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||