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PEN2 antibody

Principal name

PEN2 antibody

Alternative names for PEN2 antibody

PSENEN, MDS033, Gamma-secretase subunit PEN-2, Presenilin enhancer protein 2

SwissProt ID

Q5G235 (Bovin), Q6QI68 (Rat), Q9CQR7 (Mouse), Q9NZ42 (Human)

Gene ID

55851 (PSENEN), 66340 (Psenen), 292788

Ncbi ID

9606, NP_758844.1, NP_758844, NM_025498, NM_172341, NP_079774.1, NM_001008764

Available reactivities

Hu (Human), Hst (Hamster), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)

Background of PEN2 antibody

PEN2 (gamma-secretase subunit PEN-2 or presenilin enhancer protein 2) is an essential subunit of the gamma-secretase complex which is an endoprotease complex responsible for catalyzing the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Gamma-secretase complex is composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PSENEN/PEN2. PEN2 represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity. PEN2 is a multi-pass membrane protein predomintly localized in the endoplasmic reticulum and in the Golgi stack membrane/cis-Golgi. Defects in PEN2 have been linked to familial acne inversa type 2 (ACNINV2), a chronic relapsing inflammatory disease of the hair follicles which is primarily characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars.

Detection limit for recombinant GST tagged PSENEN is approximately 0.1ng/ml as a capture antibody.

H00055851-M01

Immunofluorescence of K562 cells with Rabbit anti PEN2 (AP01104PU-N)

AP01104PU-N

Western blot analysis of whole cell lysates from K562 human erythromyeloblastoid cells probed with Rabbit anti Human PEN2 (AP01104PU-N) at 0.5(A), 1(B) and 2(C) µg/ml.

AP01104PU-N

Western blot analysis in Rat brain homogenate ( 15 µg ) . Overnight incubation PVDF membrane with PSENEN polyclonal antibody ( Cat # PAB0141 ) ( 15 µg/ml ) in PBS containing 1% PBS. Horesradish peroxidase-conjugated anti-rabbit ( 0.5 µg/ml ; GE-Biosciences ) was used for detection.

PAB0141

Immunofluorescence of PSENEN in K-562 cells with PSENEN polyclonal antibody ( Cat # PAB13229 ) at 20 µg/ml.

PAB13229

Western blot analysis of PSENEN in K-562 cell lysate with PSENEN polyclonal antibody ( Cat # PAB13229 ) at ( A ) 0.5, ( B ) 1, and ( C ) 2 µg/ml.

PAB13229

Immunohistochemistry of PSENEN in rat lung tissue with PSENEN polyclonal antibody ( Cat # PAB13230 ) at 2.5 µg/ml.

PAB13230

Western blot analysis of PSENEN in A-20 cell lysate with PSENEN polyclonal antibody ( Cat # PAB13230 ) at ( A ) 0.5 and ( B ) 1 µg/ml.

PAB13230

Human Muscle; WB Suggested Anti-PSENEN Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Muscle; PSENEN antibody - C-terminal region (ARP59439_P050) in Human Muscle cells using Western Blot

ARP59439_P050

Human Thymus (formalin-fixed, paraffin-embedded) stained with PSENEN antibody AP22868PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AP22868PU-N

Human Placenta (formalin-fixed, paraffin-embedded) stained with PSENEN antibody AP22868PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AP22868PU-N

Human Adrenal (formalin-fixed, paraffin-embedded) stained with PSENEN antibody AP22868PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AP22868PU-N

Western blot analysis of PEN2 in A-20 cell lysate with AP22868PU-N at (A) 0.5 and (B) 1 µg/ml.

AP22868PU-N

Mouse Heart; WB Suggested Anti-Psenen Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Heart; Psenen antibody - N-terminal region (ARP59440_P050) in Mouse Heart cells using Western Blot

ARP59440_P050

TP303272

Western blot analysis of PEN2 in K562 cell lysate with PEN2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.

TA306367

Immunofluorescence of PEN2 in K562 cells with PEN2 antibody at 20 ug/mL.

TA306367

Western blot analysis of PEN2 in A-20 cell lysate with PEN2 antibody at (A) 0.5 and (B) 1 ug/ml.

TA306368

Immunohistochemistry of PEN2 in rat lung tissue with PEN2 antibody at 2.5 ug/ml.

TA306368

Immunofluorescence of PEN2 in Rat Lung cells with PEN2 antibody at 20 ug/mL.

TA306368

Western Blot: PEN2 Antibody [TA336624] - Analysis of PEN2 on human placenta.

TA336624

WB Suggested Anti-PSENEN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Muscle

TA342614

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP303272	PEN2
	Recombinant protein of human presenilin enhancer 2 homolog (C. elegans) (PSENEN)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / $748.00
				OriGene Technologies, Inc.

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