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PDK1 antibody

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Principal name

PDK1 antibody

Alternative names for PDK1 antibody

Pyruvate dehydrogenase kinase isoform 1, Pyruvate dehydrogenase [lipoamide] kinase isozyme 1 mitochondrial

SwissProt ID

Q15118 (Human), Q63065 (Rat), Q8BFP9 (Mouse)

Gene ID

5163 (PDK1)

Ncbi ID

9606, NP_002601.1, NP_002601

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Bov (Bovine), African clawed frog, Mky (Monkey), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)

Background of PDK1 antibody

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Sarbassov DD, Guertin DA, Ali SM, Sabatini DM. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005 Feb 18;307(5712):1098-101.

H00005163-M01 - PDK1
Detection limit for recombinant GST tagged PDK1 is approximately 30ng/ml as a capture antibody.
H00005163-M02 - PDK1
Detection limit for recombinant GST tagged PDK1 is approximately 3ng/ml as a capture antibody.
PAB4561 - PDK1
The PDK1 polyclonal antibody ( Cat # PAB4561 ) is used in Western blot to detect PDK1 in mouse kidney tissue lysate .
PAB6898 - PDK1
PDK1 polyclonal antibody ( Cat # PAB6898 ) ( 1 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
AR09115PU-L - PDK1
AR09115PU-L - PDK1
PDK1, 29-436 aa, His-tagged: 15% SDS-PAGE (3 µg)
AP13579PU-N - PDK1
The anti-PDK1 Pab is used in Western blot to detect PDK1 in mouse kidney tissue lysate.
PAB12077 - PDK1
Western blot analysis of PDK1 in human heart lysate with PDK1 polyclonal antibody at 2 µg/ml ( Cat# PAB12077 ).
PAB12078 - PDK1
Western blot analysis of PDK1 in human heart lysate with PDK1 polyclonal antibody at 0.5 µg/ml ( Cat# PAB12078 ).
AM09090PU-N - PDK1
ICC/IF analysis of PDK1 in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human PDK1 antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green).
AM09090PU-N - PDK1
Flow cytometry analysis of PDK1 in Balb/3T3 cell line, staining at 2-5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
AM09090PU-N - PDK1
Western blot analysis: The extracts of rat heart (60 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDK1 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
ARP31776_P050 - PDK1
Hum. Adult Placenta; Host: Rabbit. Target Name: CHAD . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; PDK1 antibody - middle region (ARP31776_P050) in Hum. Adult Placenta cells using Western Blot
ARP31776_P050 - PDK1
Human Jurkat; Host:Rabbit. Target Name:PDK2. Sample Tissue:Human Jurkat. Antibody Dilution: 1.0ug/ml. PDK1 is supported by BioGPS gene expression data to be expressed in Jurkat.; PDK1 antibody - middle region (ARP31776_P050) in Human Jurkat cells using Western Blot
ARP31776_P050 - PDK1
Human MCF-7; WB Suggested Anti-PDK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: MCF7 cell lysate; PDK1 antibody - middle region (ARP31776_P050) in Human MCF-7 cells using Western Blot
AP22886PU-N - PDK1
Skeletal muscle. Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP22886PU-N - PDK1
Western Blot Analysis of PDK1: Lane 1: MWMLane 2: HeLaLane 3: Rat BrainLane 4: Mouse BrainLane 5: VeroLane 6: ESK-4Lane 7: RK-13Lane 8: MDCK
AM06566SU-N - PDK1
Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using PDK1 antibody Cat.-No AM06566SU-N with DAB staining.
AM06566SU-N - PDK1
Immunofluorescence analysis of HELA cells using PDK1 antibody Cat.-No AM06566SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
AM06566SU-N - PDK1
Western blot analysis using PDK1 antibody Cat.-No AM06566SU-N against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.
AM06566SU-N - PDK1
Flow Cytometric analysis of Lovo cells using PDK1 antibody Cat.-No AM06566SU-N (green) and negative control (purple).
CPA1868-100ul - PDK1
Western blot analysis of PDK1 expression in LNCaP (A); A431 (B) whole cell lysates.
TP305127 - PDK1
TA301454 - PDK1
Detection of PDK1 in human heart lysate at 2 ug/ml
TA301454 - PDK1
Western Blot: Analysis of HeLa lysates using PDK1 antibody
TA301454 - PDK1
Immunocytochemistry/Immunofluorescence: PDK1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
TA301455 - PDK1
Detection of PDK1 in human heart lysate at 0.5 ug/ml.
TA303069 - PDK1
TA303069 (1µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA326285 - PDK1
Western blot analysis of extracts from Hela cells, using PDK1 antibody.The lane on the right is treated with the synthesized peptide.
TA329402 - PDK1
WB Suggested Anti-PDK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: MCF7 cell lysate
TA329402 - PDK1
2Hum. Adult Placenta; Host: Rabbit; Target Name: CHAD; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0ug/ml
TA329402 - PDK1
Host:Rabbit; Target Name:PDK2; Sample Tissue:Human Jurkat; Antibody Dilution: 1.0ug/mlPDK1 is supported by BioGPS gene expression data to be expressed in Jurkat
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDK1 antibody
Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using PDK1 antibody Cat.-No AM06566SU-N with DAB staining. Mouse IgG1 4A11 Ascites Hu, Mky, Rt E, F, ICC/IF, P, WB
0.1 ml / $410.00
  OriGene Technologies GmbH
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  1. Primary Antibody (1)
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  1. Monoclonal (1)
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  1. PDK1 (1)
Reactivity
  1. Human (Hu) (1)
  2. Monkey (Mky) (1)
  3. Rat (Rt) (1)
Applications
  1. Enzyme Immunoassay (E) (1)
  2. Flow cytometry (F) (1)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  4. Paraffin Sections (P) (1)
  5. Western blot / Immunoblot (WB) (1)
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  1. Ascites (1)
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  1. Mouse (1)
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  1. Flow cytometry (F) (1)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  3. Paraffin Sections (P) (1)
  4. Western blot / Immunoblot (WB) (1)
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