PASK antibody
Principal name
PASK antibody
Alternative names for PASK antibody
PAS-kinase, PASKIN, KIAA0135, PAS domain-containing serine/threonine-protein kinase
Ncbi ID
9606, NP_055963.2, NP_055963, NP_543126.2, NM_080850, NM_015148
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of PASK antibody
- Kit Component:
- - KN312836G1, Pask gRNA vector 1 in pCas-Guide vector
- KN312836G2, Pask gRNA vector 2 in pCas-Guide vector
- KN312836D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection limit for recombinant GST tagged PASK is approximately 0.03ng/ml as a capture antibody.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PASK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500826)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PASK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500826)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PASK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500826)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PASK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500826)
Anti-PASK mouse monoclonal antibody (TA500826) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PASK(RC209096).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PASK (RC209096, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PASK.
Flow cytometric Analysis of Jurkat cells, using anti-PASK antibody(TA500826),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-PASK antibody(TA500826),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-PASK mouse monoclonal antibody (TA500878) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PASK(RC209096).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PASK (RC209096, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PASK.
Anti-PASK mouse monoclonal antibody (TA500885) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PASK(RC209096).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PASK (RC209096, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PASK.
Sample (30ug whole cell lysate). A:Raji . 5% SDS PAGE. TA308119 diluted at 1:1000
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309096 | PASK |
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| Recombinant protein of human PAS domain containing serine/threonine kinase (PASK) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$788.00
|
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| OriGene Technologies, Inc. | |||||