PAPSS2 antibody
Principal name
PAPSS2 antibody
Alternative names for PAPSS2 antibody
ATPSK2, PAPS synthase 2, PAPSS 2, Sulfurylase kinase 2, SK2, Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Gene ID
9060 (PAPSS2)
Ncbi ID
9606, NP_001015880, NP_001015880.1, NM_001015880, NP_004661, NM_004670
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP)
Background of PAPSS2 antibody
Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008].
Immunoperoxidase of monoclonal antibody to PAPSS2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to PAPSS2 on A-431 cell. [antibody concentration 10 ug/ml]
Western blot analysis of PAPSS2 over-expressed 293 cell line, cotransfected with PAPSS2 Validated Chimera RNAi ( Cat # H00009060-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PAPSS2 monoclonal antibody (M07), clone 2A8 (Cat # H00009060-M07 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
PAPSS2 monoclonal antibody (M07), clone 2A8 Western Blot analysis of PAPSS2 expression in A-431 ( Cat # L015V1 ).
PAPSS2 monoclonal antibody (M07), clone 2A8. Western Blot analysis of PAPSS2 expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot analysis of PAPSS2 expression in transfected 293T cell line by PAPSS2 monoclonal antibody (M07), clone 2A8.Lane 1: PAPSS2 transfected lysate(69.5 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged PAPSS2 is approximately 0.03ng/ml as a capture antibody.
Immunoperoxidase of purified MaxPab antibody to PAPSS2 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to PAPSS2 on HeLa cell. [antibody concentration 10 ug/ml]
PAPSS2 MaxPab polyclonal antibody. Western Blot analysis of PAPSS2 expression in human liver.
Western Blot analysis of PAPSS2 expression in transfected 293T cell line (H00009060-T01) by PAPSS2 MaxPab polyclonal antibody.Lane 1: PAPSS2 transfected lysate(67.54 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PAPSS2 polyclonal antibody ( Cat # PAB3306 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The PAPSS2 polyclonal antibody ( Cat # PAB3306 ) is used in Western blot to detect PAPSS2 in Jurkat cell lysate.
Western Blot analysis of PAPSS2 expression in transfected 293T cell line (H00009060-T02) by PAPSS2 MaxPab polyclonal antibody.Lane 1: PAPSS2 transfected lysate(69.50 KDa).Lane 2: Non-transfected lysate.
PAPSS2 MaxPab rabbit polyclonal antibody. Western Blot analysis of PAPSS2 expression in HepG2.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
The anti-PAPSS2 Pab is used in Western blot to detect PAPSS2 in Jurkat cell lysate.
Immunoperoxidase of purified MaxPab antibody to PAPSS2 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to PAPSS2 on HeLa cell. [antibody concentration 10 ug/ml]
PAPSS2 MaxPab polyclonal antibody. Western Blot analysis of PAPSS2 expression in human liver.
Western Blot analysis of PAPSS2 expression in transfected 293T cell line (H00009060-T01) by PAPSS2 MaxPab polyclonal antibody.Lane 1: PAPSS2 transfected lysate(67.54 KDa).Lane 2: Non-transfected lysate.
Human 721_B; Host:Rabbit. Target Name:PAPSS2. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. There is BioGPS gene expression data showing that PAPSS2 is expressed in 721_B.; PAPSS2 antibody - C-terminal region (ARP48642_P050) in Human 721_B cells using Western Blot
Human HeLa; Host:Rabbit. Target Name:PAPSS2. Sample Tissue:Human Hela. Antibody Dilution: 1.0ug/ml. PAPSS2 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.; PAPSS2 antibody - C-terminal region (ARP48642_P050) in Human HeLa cells using Western Blot
Human 293T; WB Suggested Anti-PAPSS2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate; PAPSS2 antibody - C-terminal region (ARP48642_P050) in Human 293T cells using Western Blot
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PAPSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807109)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PAPSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807109)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PAPSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807109)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PAPSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807109)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAPSS2 (RC200551, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAPSS2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAPSS2 (RC200551, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAPSS2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAPSS2 (RC200551, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAPSS2.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PAPSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807112)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PAPSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807130)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PAPSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807130)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PAPSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807130)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAPSS2 (RC200551, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAPSS2.
WB Suggested Anti-PAPSS2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 293T cell lysate
Host: Rabbit; Target Name: PAPSS2; Sample Tissue: Human Hela; Antibody Dilution: 1.0 ug/ml.PAPSS2 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells
Host: Rabbit; Target Name: PAPSS2; Sample Tissue: Human 721_B; Antibody Dilution: 1.0 ug/ml.There is BioGPS gene expression data showing that PAPSS2 is expressed in 721_B
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP15046PU-N | PAPSS2 (C-term) antibody |
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Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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