PAPOLA antibody
Principal name
PAPOLA antibody
Alternative names for PAPOLA antibody
PAP-alpha, Poly(A) polymerase alpha, Polynucleotide adenylyltransferase alpha
Ncbi ID
9606, NP_116021, NP_116021.2, NP_035242.1, NM_011112, NM_032632
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)
Background of PAPOLA antibody
- Kit Component:
- - KN312794G1, Papola gRNA vector 1 in pCas-Guide vector
- KN312794G2, Papola gRNA vector 2 in pCas-Guide vector
- KN312794D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Rapti, A., et al. Mol. Cell. Biochem. 340 (1-2), 91-96 (2010)
Nan, H., et al. J. Invest. Dermatol. 129(9):2250-2257(2009)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Dettwiler, S., et al. J. Biol. Chem. 279(34):35788-35797(2004)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma reacted with AP53159PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of PAP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of PAP Antibody (Center) Cat.-No AP53159PU-N in Jurkat cell line lysates (35ug/lane). This demonstrates the PAP antibody detected the PAP protein (arrow).
WB using the antibody against PAPOLA diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.
Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against human PAPOLA. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:22,500.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA347261 | PAPOLA antibody |
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| Rabbit Polyclonal PAPOLA Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E |
0.1 ml /
$285.00
|
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AP53159PU-N | PAPOLA (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
$415.00
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|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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