NOTCH3 antibody
Principal name
NOTCH3 antibody
Alternative names for NOTCH3 antibody
Notch-3, Neurogenic locus notch homolog protein 3
Gene ID
4854 (NOTCH3)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of NOTCH3 antibody
- Kit Component:
- - KN224711G1, NOTCH3 gRNA vector 1 in pCas-Guide vector
- KN224711G2, NOTCH3 gRNA vector 2 in pCas-Guide vector
- KN224711D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Dang L, Fan X, Chaudhry A, Wang M, Gaiano N, Eberhart CG. Notch3 signaling initiates choroid plexus tumor formation. Oncogene. 2005 Sep 26; [Epub ahead of print]
PMID: 16186803
Detection limit for recombinant GST tagged NOTCH3 is approximately 0.03ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NOTCH3 is approximately 3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NOTCH3 is approximately 10ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with NOTCH3 polyclonal antibody ( Cat # PAB1984 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP08772PU-N at 5 µg/ml staining of NOTCH3 in Human Placenta tissue by Immunohistochemistry (FFPE)
(4ug/ml) staining of paraffin embedded Human Hippocampus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Predicted band size: 244 kDa . Positive control: Human colon cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human colon cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using NOTCH3 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP08772PU-N | NOTCH3 (C-term) antibody |
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Goat | IgG | Aff - Purified | Bt, Elephant, Hu, Mky, Ms, Rt | E, P |
50 µg /
$425.00
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| OriGene Technologies GmbH | |||||||