Home » Target » NOTCH2 antibody

NOTCH2 antibody

Principal name

NOTCH2 antibody

Alternative names for NOTCH2 antibody

Notch 2, hN2, Neurogenic locus notch homolog protein 2

SwissProt ID

O35516 (Mouse), Q04721 (Human), Q9QW30 (Rat)

Gene ID

4853 (NOTCH2), 29492 (Notch2), 18129

Ncbi ID

9606, NP_001186930.1, NP_077334.1, NP_077719, NP_035058.2

Available reactivities

Hu (Human), Can (Canine), Rt (Rat), Mky (Monkey), Ms (Mouse), Chimp (Chimpanzee), Bov (Bovine), Hst (Hamster), Por (Porcine), Prim (Primate), Rb (Rabbit), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F)

Background of NOTCH2 antibody

NOTCH2 is a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch sigling network is an evolutiorily conserved intercellular sigling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular sigling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. NOTCH2 is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. NOTCH2 functions as a receptor for membrane bound ligands, and may play a role in vascular, rel and hepatic development.This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch sigling network is an evolutiorily conserved intercellular sigling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular sigling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, rel and hepatic development. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

Formalin-Fixed Paraffin-Embedded Human Kidney stained with NOTCH2 antibody Cat.-No AP07611SU-N

AP07611SU-N

Western blot using anti-Notch 2 (intra) antibody shows detection of a band at ~110 kD corresponding to active Notch 2 protein (arrowhead). Western Blot analysis was performed for Notch 2 expression using 100 µg of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2).
Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection.
Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points.
The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1/400 dilution. ECL was used as visualization method.

AP07611SU-N

Figure 1. Staining NOTCH2 in Liver by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.

AP07864SU-N

Immunohistochemical staining with NOTCH2 polyclonal antibody ( Cat # PAB10293 ) was diluted 1 : 500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

PAB10293

Immunohistochemical staining with NOTCH2 polyclonal antibody ( Cat # PAB10294 ) was diluted 1 : 500 to detect NOTCH 2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

PAB10294

Western blot using NOTCH2 polyclonal antibody ( Cat # PAB10294 ) ( intra ) antibody shows detection of a band at ~110 kDa correspondingto active NOTCH2 protein ( arrowhead ).Western Blot analysis was performed for NOTCH2 expression using 100 µg of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active NOTCH2 ( intra cellular NOTCH2 ).Lanes 1-3 contain lysate 24 h ( 1 ), 48 h ( 2 ), and 72 h ( 3 ) post transfection.Lanes 4-6 are the corresponding control cells ( untransfected ) taken at similar timepoints.The band at about 110 kD represents active NOTCH2.This band is not seen in the control cell.The intracellular domain of NOTCH2 has a predicted band size of 110kD, corresponding to this band.Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milkand probed with NOTCH2 polyclonal antibody at a 1 : 400 dilution.ECL was used as visualization method.

PAB10294

Human 721_B; WB Suggested Anti-NOTCH2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. There is BioGPS gene expression data showing that NOTCH2 is expressed in 721_B.; NOTCH2 antibody - middle region (ARP32723_P050) in Human 721_B cells using Western Blot

ARP32723_P050

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Notch 2 (Cleaved-Asp1733) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells, treated with TNF-a 20ng/ml 30', using Notch 2 (Cleaved-Asp1733) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Jurkat cells, treated with etoposide 25uM 24h, using NOTCH2 (Cleaved-Ala1734) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Jurkat cells, treated with etoposide 25uM 24h, using NOTCH2 (Cleaved-Val1697) Antibody. The lane on the right is treated with the synthesized peptide.

Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with NOTCH2 antibody AP22760PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AP22760PU-N

Human Liver (formalin-fixed, paraffin-embedded) stained with NOTCH2 antibody AP22760PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AP22760PU-N

Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with NOTCH2 antibody AP22760PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AP22760PU-N

Immunohistochemical analysis of NOTCH2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of NOTCH2 expression in HeLa (A); MCF7 (B); rat liver (C); NIH3T3 (D) whole cell lysates.

Western blot analysis of NOTCH2 expression in HepG2 colchicine-treated (A); Raw264.7 colchicine-treated (B); rat liver (C) whole cell lysates.

Western blot analysis of extracts from 293 cells treated with TNF-a (20ng/ml, 30mins), using Notch 2 (cleaved-Asp1733) antibody.The lane on the right is treated with the synthesized peptide.

TA311717

Immunohistochemical analysis of paraffin-embedded human brain tissue using Notch 2 (cleaved-Asp1733) antibody.The picture on the right is treated with the synthesized peptide.

TA311717

Western blot analysis of extracts from Jurkat cells, treated with etoposide (25uM, 24hours), using NOTCH2 (Cleaved-Ala1734) antibody.The lane on the right is treated with the synthesized peptide.

TA314277

Western blot analysis of extracts from Jurkat cells, treated with etoposide (25uM, 24hours), using NOTCH2 (Cleaved-Val1697) antibody.The lane on the right is treated with the synthesized peptide.

TA314278

anti-NOTCH 2 antibody was diluted 1:500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

TA319402

WB using anti-Notch 2 (intra) antibody shows detection of a band at ~110 kDa corresponding to active Notch 2 protein (arrowhead). WB analysis was performed for Notch 2 expression using human mesothelial SV40 cells lysate obtained fromtransfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected). Anti-Notch 2 was used at 1:400.

TA319403

Anti-Notch 2 antibody was diluted 1:500 to detect NOTCH 2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

TA319403

Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using NOTCH2 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification: x200)

TA322182

WB Suggested Anti-NOTCH2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysateThere is BioGPS gene expression data showing that NOTCH2 is expressed in 721_B