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NKRF antibody

Principal name

NKRF antibody

Alternative names for NKRF antibody

ITBA4, NRF, NF-kappa-B-repressing, factorTranscription factor NRF, ITBA4 protein

SwissProt ID

O15226 (Human), Q8BY02 (Mouse)

Gene ID

55922 (NKRF), 77286 (Nkrf)

Ncbi ID

NP_060014, 9606, NP_001166958.1, NP_084167.2, NM_029891, NM_017544, NM_001173488

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of NKRF antibody

NKRF is a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. NKRF localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm.This gene encodes a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. This gene encodes a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm.

General readings

Dreikhausen,U., et al., (2005) Mol. Cell. Biol. 25 (17), 7473-7483

Human HepG2; WB Suggested Anti-NKRF Antibody Titration: 2.5ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; NKRF antibody - N-terminal region (ARP39289_T100) in Human HepG2 cells using Western Blot

ARP39289_T100

Western Blot analysis of NKRF expression in transfected 293T cell line by NKRF monoclonal antibody (M06), clone 1F6.Lane 1: NKRF transfected lysate(77.7 KDa).Lane 2: Non-transfected lysate.

H00055922-M06

Western Blot analysis of NKRF expression in transfected 293T cell line (H00055922-T01) by NKRF MaxPab polyclonal antibody.Lane 1: NKRF transfected lysate(75.9 KDa).Lane 2: Non-transfected lysate.

H00055922-B01

Western Blot analysis of NKRF expression in transfected 293T cell line (H00055922-T02) by NKRF MaxPab polyclonal antibody.Lane 1: NKRF transfected lysate(75.9 KDa).Lane 2: Non-transfected lysate.

H00055922-B01P

Human Breast; Immunohistochemistry with Human Breast tissue at an antibody concentration of 5.0ug/ml using anti-NKRF antibody (ARP39290_P050); NKRF antibody - middle region (ARP39290_P050) in Human Breast cells using Immunohistochemistry

ARP39290_P050

Human Muscle; WB Suggested Anti-NKRF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Muscle; NKRF antibody - middle region (ARP39290_P050) in Human Muscle cells using Western Blot

ARP39290_P050

Western blot analysis of NKRF antibody (Center) (AP17593PU-N) in Jurkat cell line lysates (35ug/lane). NKRF (arrow) was detected using the purified Pab.

AP17593PU-N

Transfected 293T; WB Suggested Anti-NKRF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; NKRF antibody - N-terminal region (ARP35929_P050) in Transfected 293T cells using Western Blot

ARP35929_P050

Transfected 293T; WB Suggested Anti-NKRF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; NKRF antibody - N-terminal region (ARP35930_P050) in Transfected 293T cells using Western Blot

ARP35930_P050

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NKRF (RC208507, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NKRF.