NEC2 antibody
Principal name
NEC2 antibody
Alternative names for NEC2 antibody
PCSK2, Neuroendocrine convertase 2,Prohormone convertase 2, Proprotein convertase 2, KEX2-like endoprotease 2
SwissProt ID
P16519 (Human), P21661 (Mouse), P28841 (Rat), Q03333 (Pig), Q5REC2 (Ponab), Q9GLR0 (Bovin)
Ncbi ID
NP_001188457.1, 9606, NP_001124879.1, NM_002594, NP_002585, NP_032818.1, NM_012746
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of NEC2 antibody
- Kit Component:
- - KN312975G1, Pcsk2 gRNA vector 1 in pCas-Guide vector
- KN312975G2, Pcsk2 gRNA vector 2 in pCas-Guide vector
- KN312975D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
PCSK2 MaxPab rabbit polyclonal antibody. Western Blot analysis of PCSK2 expression in mouse liver.
PCSK2 MaxPab rabbit polyclonal antibody. Western Blot analysis of PCSK2 expression in human liver.
Western Blot analysis of PCSK2 expression in transfected 293T cell line (H00005126-T01) by PCSK2 MaxPab polyclonal antibody.Lane 1: PCSK2 transfected lysate(70.60 KDa).Lane 2: Non-transfected lysate.
Human HeLa; WB Suggested Anti-PCSK2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate; PCSK2 antibody - middle region (ARP59552_P050) in Human HeLa cells using Western Blot
Western blot analysis of PCSK2 Antibody (N-term) (Cat. #TA324950) in mouse cerebellum tissue lysates (35ug/lane). PCSK2 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human brain tissue reacted with PCSK2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of PCSK2 Antibody (N-term)(Cat#TA324950) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
PCSK2 Antibody (N-term) (Cat. #TA324950) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-PCSK2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Hela cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324950 | NEC2 (N-term) antibody |
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| Rabbit polyclonal PCSK2 Antibody (N-term) | |||||||
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Rabbit | Hu, Ms | WB |
0.4 ml /
$415.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
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| SP1377P | NEC2 antibody |
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Rabbit | Serum | Hu, Mky, Rt | WB |
0.1 ml /
$590.00
|
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP322498 | NEC2 |
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| Recombinant protein of human proprotein convertase subtilisin/kexin type 2 (PCSK2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419211 | PCSK2 overexpression lysate |
|
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0.1 mg /
$495.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214318L2V | Lenti ORF particles, Pcsk2 (GFP-tagged ORF) - Rat proprotein convertase subtilisin/kexin type 2 (Pcsk2), 200 uL, >10^7 TU/mL |
|
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200 µl /
$1,380.00
|
|
| OriGene Technologies, Inc. | ||
| RR214318L1V | Lenti ORF particles, Pcsk2 (Myc-DDK-tagged ORF) - Rat proprotein convertase subtilisin/kexin type 2 (Pcsk2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,240.00
|
|
| OriGene Technologies, Inc. | ||