NDUFS2 antibody
Principal name
NDUFS2 antibody
Alternative names for NDUFS2 antibody
Complex I-49kD, CI-49kD, NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial, NADH-ubiquinone oxidoreductase 49 kDa subunit
Ncbi ID
9606, NP_004541, NP_001159631, NP_001159631.1, NP_694704.1, NM_153064, NM_004550, NM_001166159
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)
Background of NDUFS2 antibody
- Kit Component:
- - KN310855G1, Ndufs2 gRNA vector 1 in pCas-Guide vector
- KN310855G2, Ndufs2 gRNA vector 2 in pCas-Guide vector
- KN310855D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Saada A, Vogel RO, Hoefs SJ, van den Brand MA, Wessels HJ, Willems PH, Venselaar H, Shaag A, Barghuti F, Reish O, Shohat M, Huynen MA, Smeitink JA, van den Heuvel LP, Nijtmans LG. Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease. American journal of human genetics 2009 Jun 84 (6): 718-27.
PMID: 19463981
NDUFS2 MaxPab polyclonal antibody. Western Blot analysis of NDUFS2 expression in human kidney.
Western Blot analysis of NDUFS2 expression in transfected 293T cell line (H00004720-T01) by NDUFS2 MaxPab polyclonal antibody.Lane 1: NDUFS2 transfected lysate(50.93 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of NDUFS2 transfected lysate using anti-NDUFS2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NDUFS2 purified MaxPab mouse polyclonal antibody (B01P) (H00004720-B01P).
Immunohistochemistryanalysis in formalin fixed and paraffin embedded hepatocarcinoma reacted with NDUFS2 Antibody (Center) Cat.-No AP52844PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of the NDUFS2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of NDUFS2 Antibody (Center) Cat.-No AP52844PU-N in Y79,A375,Hela cell line and mouse brain,cerebellum tissue lysates (35ug/lane). This demonstrates the NDUFS2 antibody detected the NDUFS2 protein (arrow).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NDUFS2 mouse monoclonal antibody. (TA802325) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (TA802325) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NDUFS2 mouse monoclonal antibody. (TA802325) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (TA802325) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (TA802325) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NDUFS2 mouse monoclonal antibody. (TA802325) Dilution: 1:150
Western blot analysis of A549 cell lysate (35ug) by using anti-NDUFS2 monoclonal antibody. Dilution: 1:500
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFS2 (RC203485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFS2.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802340)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802340)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802340)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802340)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802340)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802340)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802340)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802340)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802340)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802340)
Western blot analysis of A549 cell lysate (35ug) by using anti-NDUFS2 monoclonal antibody. Dilution: 1:500
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFS2 (RC203485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFS2.
Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA307995 diluted at 1:10000.
Sample (30 ug of whole cell lysate). A: 293T. B: A431 . C: JurKat. D: Raji. 10% SDS PAGE. TA307995 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded Colon ca, using NDUFS2(TA307995) antibody at 1:250 dilution.
NDUFS2 antibody detects NDUFS2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37â for 30 min. Green: NDUFS2 protein stained by NDUFS2 antibody (TA307995) diluted at 1:500. Blu
(0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA307995 | NDUFS2 antibody |
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| Rabbit Polyclonal antibody to NDUFS2 (NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)) | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 ml /
$415.00
|
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA802325 | NDUFS2 antibody |
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| NDUFS2 mouse monoclonal antibody, clone OTI2G2 (formerly 2G2) | |||||||
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Mouse | IgG1 | OTI2G2 | Purified | Hu | P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| TA802340 | NDUFS2 antibody |
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| NDUFS2 mouse monoclonal antibody, clone OTID5 (formerly D5) | |||||||
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Mouse | IgG1 | OTID5 | Purified | Hu | P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
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