NDUFA4 antibody
Principal name
NDUFA4 antibody
Alternative names for NDUFA4 antibody
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4
Ncbi ID
9606, NP_002480, NP_002480.1, NP_035016.1, NM_010886, NM_002489
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of NDUFA4 antibody
- Kit Component:
- - KN310827G1, Ndufa4 gRNA vector 1 in pCas-Guide vector
- KN310827G2, Ndufa4 gRNA vector 2 in pCas-Guide vector
- KN310827D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kim J.W., Lee Y., et al. (1997), Biochem. Mol. Biol. Int. 43:669-675
Western blot: analysis of NDUFA4 antibody (Cat.-No.: AP20126PU-N) staining in extracts from RAW264.7 cells.
Western blot analysis of NDUFA4 antibody (Cat.-No.: AP20126PU-N) at 1/500 dilution. Lane 1: HEK293T whole cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: H9C2 whole cell lysate.
Immunohistochemical analysis of NDUFA4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of NDUFA4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of NDUFA4 expression in HeLa (A) whole cell lysates.
Western blot analysis of extracts from HepG2 cells and RAW264.7 cells, using NDUFA4 antibody.The lane on the right is treated with the synthesized peptide.
NDUA4 Antibody (C-term) (Cat. #TA328114) western blot analysis in SW480,Hela cell line lysates (35ug/lane).This demonstrates the NDUA4 antibody detected the NDUA4 protein (arrow).
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-4: SKOV3 cells, human fetal liver tissue, human fetal brain tissue, Hela cells, Primary antibody: (NDUFA4 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (NDUFA4 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (NDUFA4 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310482 | NDUFA4 |
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| Recombinant protein of human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9kDa (NDUFA4), nuclear gene encoding mitochondrial protein | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
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| OriGene Technologies, Inc. | |||||