Myelin Basic Protein antibody
Principal name
Myelin Basic Protein antibody
Alternative names for Myelin Basic Protein antibody
MBP, Myelin membrane encephalitogenic protein
SwissProt ID
P02686 (Human), P02687 (Bovin), P02688 (Rat), P04370 (Mouse), P15720 (Chick), P81558 (Pig)
Ncbi ID
NP_001020252.1, 9606, NP_001020252, NP_001020272, NP_001020271, NP_001020263.1, NM_001025081, NM_001025090, NP_001020261, NP_001020263, NP_001020416.1, NM_001025245, NM_010777, NM_001025255, NM_001025256, NM_001025258, NM_001025259, NM_017026, NM_001025291, NM_001025292, NM_001025293, NM_001025294, NM_001025289, NM_001025100, NM_001025092, NM_002385
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Radioimmunoassay (R), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Flow Cytometry (F), Immunoprecipitation (IP)
Background of Myelin Basic Protein antibody
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelition of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelition, maybe as components of transcriptiol complexes, and may also be involved in sigling pathways in T-cells and neural cells. Differential splicing events combined with optiol post-translatiol modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. MBP induces T-cell proliferation.The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRs arise from the long MBP gene (otherwise called 'Golli-MBP') that contains 3 additiol exons located upstream of the classic MBP exons. Altertive splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-termil Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
General readings
Nye S.H., et al (1995). Mol. Immunol. 32:1131-1141Pribyl T.M., et al (1993). Proc. Natl. Acad. Sci. U.S.A. 90:10695-10699
Immunohistochemistry : BM017 BMP antibody staining of Formalin-Fixed Paraffin-Embedded Human Brain, Cortex at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry : BM017 BMP antibody staining of Formalin-Fixed Paraffin-Embedded Human Brain, Cerebellum at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Mouse Brain Tissue lysate probed with Rat anti MBP antibody
Staining of white matter in human brain with Mouse anti MBP
Dot blot analysis of anti-Phospho-MBP pTyr203 Antibody (Cat.#AP12968PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Immunohistochemical analysis of paraffin-embedded human liver sections using MBP polyclonal antibody ( Cat # PAB7988 ) .
Western blot analysis of rat brain tissue lysate. Using MBP polyclonal antibody ( Cat # PAB7988 ) .
Dot blot analysis of MBP (phospho Y203) polyclonal antibody ( Cat # PAB8164 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.
Immunohistochemistry on human brain with brain trauma ( car accident ), using MBP polyclonal antibody ( Cat # PAB14324 ) at a dilution of 1 : 2000. Courtesy of Dr. Wei-Ping Gai, The Flinders University of South Australia.
Human Brain; WB Suggested Anti-MBP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; MBP antibody - middle region (ARP56223_P050) in Human Brain cells using Western Blot
Human brain; . Sample Type :. Human brain stem cells. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. MBP: Red DAPI:Blue. Gene Name :. MBP. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; MBP antibody - middle region (ARP56224_P050) in Human brain cells using Immunohistochemistry
Human brain; WB Suggested Anti-MBP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; MBP antibody - middle region (ARP56224_P050) in Human brain cells using Western Blot
Rat brain; . Amount and Sample Type :. 500 ug rat brain homogenate. Amount of IP Antibody :. 6 ug. Primary Antibody :. MBP. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:5000. Gene Name :. MBP. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; MBP antibody - middle region (ARP56224_P050) in Rat brain cells using Immunoprecipitation
Human NT2; IP Suggested Anti-MBP Antibody Positive Control: NT2 CELL/BRAIN TISSUE; MBP antibody - middle region (ARP56224_P050) in Human NT2 cells using Immunoprecipitation
Immunofluorescence. Rat mixed neuron/glial cultures stained with Chicken Myelin Basic Protein antibody (Green). Blue is a DNA stain. Note that the MBP antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative. specifically, and that the staining is concentrated in the cell bodies.
Brain, cerebellum, white matter, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemical analysis of paraffin embedded Human tissue sections (liver) using MBP antibody
Western blot analysis of rat brain tissue lysis using MBP antibody
A tissue section through an adult Mouse brain showing MBP (red staining) of white matter tracts adjacent to the hippocampal formation. Green staining is autofluorescence from Green Fluorescent Protein (GFP) expressed in this transgenic Mouse.
Immunohistochemical analysis of paraffin-embedded brain tissues using MBP mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded cerebellum tissues using MBP mouse mAb with DAB staining.
Immunofluorescence analysis of MSCS cells using MBP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using MBP mAb against human MBP (AA: 1-197) recombinant protein. (Expected MW is 70 kDa)
Western blot analysis using MBP mAb against HEK293 (1) and MBP-hIgGFc transfected HEK293 (2) cell lysate.
Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)
Flow cytometric analysis of HepG2 cells using MBP mouse mAb (green) and negative control (red).
Western blot of rat cortex homogenate showing specific immunolabeling of the ~ 18 & 22k MBP protein.
Rat mixed neuron/glial cultures stained with chicken anti-Myelin Basic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.
Anti-Myelin Basic Protein antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Myelin Basic Protein antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Predicted band size: 33 kDa . Positive control: Rat brain, Mouse brain and Rat hippocampus tissue lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: Rat brain tissue lysate Lane2: Mouse brain tissue lysate Lane3: Rat hippocampus tissue lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of extracts of various cell lines, using MBP antibody.
Immunohistochemistry staining of Paraffin Brain tissue by Myelin Basic Protein antibody (dilution: 1:50 - 1:200; visualization of staining: Cytoplasmic)
WB Suggested Anti-MBP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain
WB Suggested Anti-MBP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain
Sample Type :; Human brain stem cellsPrimary Antibody Dilution :; 1:500Secondary Antibody :; Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :; 1:1000Color/Signal Descriptions :; MBP: Red DAPI:BlueGene Name :; MBPSubmitted by :; Dr. Yuzhi Chen, University of Arkansas for Medical Science
Amount and Sample Type :; 500 ug rat brain homogenate; Amount of IP Antibody :; 6 ug; Primary Antibody :; MBP; Primary Antibody Dilution :; 1:500; Secondary Antibody :; Goat anti-rabbit Alexa-Fluor 594; Secondary Antibody Dilution :; 1:5000; Gene Name :; MBP; Submitted by :; Dr. Yuzhi Chen, University of Arkansas for Medical Science
IP Suggested Anti-MBP Antibody Positive Control: NT2 CELL/BRAIN TISSUE
Western Blot: Myelin Basic Protein Antibody [TA336547] - WB analysis of Myelin Basic Protein (MBP) in 10ug of normal brain lysate with antibody TA336547 at 1:500 dilution.
Western Blot: Myelin Basic Protein Antibody (2H9) [TA336919] - Western blot analysis using Myelin Basic Protein mAb against HEK293 (1) and Myelin Basic Protein-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Myelin Basic Protein Antibody (2H9) [TA336919] - Western blot analysis using Myelin Basic Protein mAb against human Myelin Basic Protein (AA: 1-197) recombinant protein. (Expected MW is 70 kDa)
ELISA: Myelin Basic Protein Antibody (2H9) [TA336919] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).
Immunohistochemistry-Paraffin: Myelin Basic Protein Antibody (2H9) [TA336919] - Immunohistochemical analysis of paraffin-embedded brain tissues using Myelin Basic Protein mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: Myelin Basic Protein Antibody (2H9) [TA336919] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using Myelin Basic Protein mouse mAb with DAB staining.
Immunocytochemistry/Immunofluorescence: Myelin Basic Protein Antibody (2H9) [TA336919] - Immunofluorescence analysis of MSCS cells using Myelin Basic Protein mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with A
Flow Cytometry: Myelin Basic Protein Antibody (2H9) [TA336919] - Flow cytometric analysis of HepG2 cells using Myelin Basic Protein mouse mAb (green) and negative control (red).
Western blot analysis of extract from Rat brain, Mouse brain and Rat hippocampus Tissue using MBP Antibody
Immunofluorescence image of rat brain (8 um sagittal section) stained with the SMI 94 antibody (red), GFAP (green) and Hoechst (blue). Photo courtesy of Molecular Expressions.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA336919 | Myelin Basic Protein antibody |
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| Mouse Monoclonal MBP Antibody (2H9) | |||||||
![Western Blot: Myelin Basic Protein Antibody (2H9) [TA336919] - Western blot analysis using Myelin Basic Protein mAb against HEK293 (1) and Myelin Basic Protein-hIgGFc transfected HEK293 (2) cell lysate.](https://m1.acris-antibodies.com/catalog/product/cache/2/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-22121-1-W.jpg) |
Mouse | IgG1 | 2H9 | Purified | Hu | E, F, ICC/IF, P, WB |
0.1 ml /
$415.00
|
| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody! |
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| AM06698SU-N | Myelin Basic Protein antibody |
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Mouse | IgG1 | 2H9 | Ascites | Hu | E, F, ICC/IF, P, WB |
0.1 ml /
$410.00
|
| OriGene Technologies GmbH | |||||||
| +6 additional images |
Highly validated antibody! |
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| TA309175 | Myelin Basic Protein antibody |
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| Chicken Anti-Myelin Basic Protein (MBP) Antibody | |||||||
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Chicken | Ig | Ig Fraction | Bov, Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
$355.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA318238 | Myelin Basic Protein antibody |
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| Myelin Basic Protein / MBP Mouse Monoclonal (N-Terminus) (V/h5) Antibody | |||||||
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Mouse | IgG1 | V/h5 | Purified | GP, Hu, Rb, Sh | P |
50 µg /
$425.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA323413 | Myelin Basic Protein antibody |
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| Anti-MBP Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
$335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA327130 | Myelin Basic Protein antibody |
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| MBP Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327706 | Myelin Basic Protein antibody |
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| Rabbit Polyclonal Myelin Basic Protein Antibody | |||||||
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Rabbit | Purified | Hu | P |
1 ml /
$975.00
|
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| OriGene Technologies, Inc. | |||||||
| TA336547 | Myelin Basic Protein antibody |
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| Rabbit Polyclonal MBP Antibody | |||||||
![Western Blot: Myelin Basic Protein Antibody [TA336547] - WB analysis of Myelin Basic Protein (MBP) in 10ug of normal brain lysate with antibody TA336547 at 1:500 dilution.](https://m1.acris-antibodies.com/catalog/product/cache/2/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB110-79873-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 mg /
$450.00
|
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| OriGene Technologies, Inc. | |||||||
| TA347839 | Myelin Basic Protein antibody |
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| Rabbit Polyclonal Anti-MBP(myelin basic protein) Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP05363PU-N | Myelin Basic Protein antibody |
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Rabbit | IgG | Purified | Hu, Rt | E, WB |
1 ml /
$330.00
|
|
| OriGene Technologies GmbH | |||||||
| AP12968PU-N | Myelin Basic Protein pTyr203 antibody |
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Rabbit | Ig | Aff - Purified | Hu |
0.4 ml /
$415.00
|
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| OriGene Technologies GmbH | |||||||
| AP23275PU-N | Myelin Basic Protein (C-term) antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, IP, P, WB |
0.1 mg /
$390.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP31809PU-N | Myelin Basic Protein antibody |
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Chicken | Ig | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 mg /
$550.00
|
|
| OriGene Technologies GmbH | |||||||
| BM017 | Myelin Basic Protein (N-term) antibody |
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Mouse | IgG1 | V/h5 | Purified | Bov, GP, Hu, Por, Rb, Sh | E, P, R |
0.2 mg /
$460.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| SM1449S | Myelin Basic Protein (36-50) antibody |
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Rat | IgG | 14 | Supernatant | Hu, Mam | C, E, WB |
2 ml /
$570.00
|
| OriGene Technologies GmbH | |||||||
| SM1450S | Myelin Basic Protein (67-74) antibody |
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Mouse | IgG1 | 26 | Supernatant | Hu, Mam | C, WB |
2 ml /
$510.00
|
| OriGene Technologies GmbH | |||||||
| SM1453S | Myelin Basic Protein (84-89) antibody |
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 |
Mouse | IgG2b | 22 | Supernatant | Hu, Mam | C, P, WB |
2 ml /
$510.00
|
| OriGene Technologies GmbH | |||||||
| SM1454 | Myelin Basic Protein (119-131) antibody |
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Mouse | IgG1 | 2 | Supernatant | Bov, GP, Hu, Rt, Sh | C, E |
2 ml /
$510.00
|
| OriGene Technologies GmbH | |||||||
| SM1455 | Myelin Basic Protein (129-138) antibody |
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Mouse | IgG2a | 1 | Supernatant | Bov, Hu, Rt | C, E |
2 ml /
$510.00
|
| OriGene Technologies GmbH | |||||||
| SM1571P | Myelin Basic Protein pThr98 antibody |
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Mouse | IgG2a | 98P/12 | Purified | Hu | C, E, WB |
0.1 mg /
$500.00
|
| OriGene Technologies GmbH | |||||||