MTHFR antibody
Principal name
MTHFR antibody
Alternative names for MTHFR antibody
Methylenetetrahydrofolate reductase
Gene ID
4524 (MTHFR)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Paraffin Sections (P)
Background of MTHFR antibody
- Kit Component:
- - KN208588G1, MTHFR gRNA vector 1 in pCas-Guide vector
- KN208588G2, MTHFR gRNA vector 2 in pCas-Guide vector
- KN208588D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection limit for recombinant GST tagged MTHFR is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to MTHFR on HeLa cell . [antibody concentration 10 ug/ml]
Detection limit for recombinant GST tagged MTHFR is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of MTHFR expression in transfected 293T cell line (H00004524-T01) by MTHFR MaxPab polyclonal antibody.Lane 1: MTHFR transfected lysate(74.50 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MTHFR expression in transfected 293T cell line (H00004524-T02) by MTHFR MaxPab polyclonal antibody.Lane 1: MTHFR transfected lysate(72.16 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical analysis of paraffin-embedded intima cancer tissues (left) and prostate tissues (right) using MTHFR mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded lung cancer using MTHFR mouse mAb with DAB staining.
Western blot analysis using MTHFR mAb against HEK293 (1) and MTHFR(AA: 339-499)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using MTHFR mouse mAb against Rat Heart cell lysate.
Red: Control Antigen (100ng);Purple: Antigen (10ng);Green: Antigen (50ng);Blue: Antigen (100ng);
Western blot analysis of MTHFR expression in HEK293 (A); Jurkat (B); A549 (C) whole cell lysates.
Sample (30 ug of whole cell lysate). A: 293T. 7.5% SDS PAGE. TA308226 diluted at 1:1000.
TA309530 (0.3µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM06617SU-N | MTHFR antibody |
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Mouse | IgG1 | 5D3 | Ascites | Hu, Rt | E, P, WB |
0.1 ml /
$410.00
|
| OriGene Technologies GmbH | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA308226 | MTHFR antibody |
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| Rabbit polyclonal antibody to MTHFR (5,10-methylenetetrahydrofolate reductase (NADPH)) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 ml /
$415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA309530 | MTHFR (369-383) antibody |
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| Goat Polyclonal Antibody against MTHFR (aa369-383) | |||||||
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | E, WB |
0.1 mg /
$360.00
|
||
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308588 | MTHFR |
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| Recombinant protein of human 5,10-methylenetetrahydrofolate reductase (NADPH) (MTHFR) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||