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MTF1 antibody

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Principal name

MTF1 antibody

Alternative names for MTF1 antibody

Transcription factor MTF-1, MRE-binding transcription factor, Metal regulatory transcription factor 1

SwissProt ID

P14908 (Yeast), Q07243 (Mouse), Q14872 (Human)

Gene ID

4520 (MTF1)

Ncbi ID

9606, NP_005946, NP_005946.2, NM_005955

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of MTF1 antibody

Kit Component:
- KN204861G1, MTF1 gRNA vector 1 in pCas-Guide vector
- KN204861G2, MTF1 gRNA vector 2 in pCas-Guide vector
- KN204861D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Chen,X., et al., (2004) J. Biol. Chem. 279 (6), 4515-4522

H00004520-M01 - MTF1
Detection limit for recombinant GST tagged MTF1 is approximately 0.3ng/ml as a capture antibody.
H00004520-M04 - MTF1
Detection limit for recombinant GST tagged MTF1 is approximately 0.03ng/ml as a capture antibody.
H00004520-M06 - MTF1
Detection limit for recombinant GST tagged MTF1 is approximately 0.03ng/ml as a capture antibody.
ARP32027_T100 - MTF1
Human HepG2; WB Suggested Anti-MTF1 Antibody Titration: 5.0ug/ml. ELISA Titer: 1:2500. Positive Control: HepG2 cell lysate. MTF1 is supported by BioGPS gene expression data to be expressed in HepG2.; MTF1 antibody - C-terminal region (ARP32027_T100) in Human HepG2 cells using Western Blot
TA805437 - MTF1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTF1 (RC204861, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTF1.
TA805438 - MTF1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTF1 (RC204861, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTF1.
TA805480 - MTF1
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MTF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
TA805480 - MTF1
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MTF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
TA805480 - MTF1
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MTF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
TA805480 - MTF1
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MTF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
TA805480 - MTF1
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MTF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
TA805480 - MTF1
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MTF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
TA805480 - MTF1
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MTF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
TA805480 - MTF1
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MTF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
TA805480 - MTF1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTF1 (RC204861, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTF1.(1:2000)
TA805483 - MTF1
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MTF1 mouse monoclonal antibody. (TA805483) Dilution: 1:150
TA805483 - MTF1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTF1 (RC204861, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTF1.
TP304861 - MTF1
TA329754 - MTF1
WB Suggested Anti-MTF1 Antibody Titration: 5.0 ug/ml; ELISA Titer: 1:2500; Positive Control: HepG2 cell lysateMTF1 is supported by BioGPS gene expression data to be expressed in HepG2
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  2. Highly Validated (1)

6 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MTF1 antibody
  MTF1 mouse monoclonal antibody, clone OTI2F3 (formerly 2F3)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MTF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150) Mouse IgG1 OTI2F3 Purified Hu P, WB
0.1 ml / $360.00
  OriGene Technologies, Inc.
+8 additional images

Highly validated antibody!

TrueMAB

MTF1 antibody
  MTF1 mouse monoclonal antibody, clone OTI1D3 (formerly 1D3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTF1 (RC204861, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTF1. Mouse IgG1 OTI1D3 Purified Hu WB
0.1 ml / $360.00
  OriGene Technologies, Inc.
 

TrueMAB

MTF1 antibody
  MTF1 mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTF1 (RC204861, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTF1. Mouse IgG2b OTI1G4 Purified Hu WB
0.1 ml / $360.00
  OriGene Technologies, Inc.
 

TrueMAB

MTF1 antibody
  MTF1 mouse monoclonal antibody, clone OTI7D6 (formerly 7D6)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MTF1 mouse monoclonal antibody. (TA805483) Dilution: 1:150 Mouse IgG2b OTI7D6 Purified Hu P, WB
0.1 ml / $360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MTF1
  Recombinant protein of human metal-regulatory transcription factor 1 (MTF1)  
MTF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

MTF1 overexpression lysate
MTF1 overexpression lysate  
0.1 mg / $295.00
  OriGene Technologies, Inc.

6 Item(s)

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Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (4)
  3. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (4)
Target
  1. MTF1 (5)
Reactivity
  1. Human (Hu) (4)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Paraffin Sections (P) (2)
Presentation
  1. Purified (4)
Host
  1. Mouse (4)
Images
  1. 1 image (5)
  2. 3+ images (1)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Paraffin Sections (P) (2)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (6)
Service
 
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