MEOX2 antibody
Principal name
MEOX2 antibody
Alternative names for MEOX2 antibody
GAX, MOX2, Growth arrest-specific homeobox, Mesenchyme homeobox 2, Homeobox protein MOX-2
Ncbi ID
9606, NP_005915, NP_005915.2, AAH17021, NM_005924, NP_032610.1, NM_008584, NM_017149
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of MEOX2 antibody
Closely related homeobox proteins, MOX-1 and MOX-2, belong to a family of nonclustered, diverged homeobox genes that are expressed in overlapping patterns in the paraxial mesoderm and its derivatives. MOX-1 and MOX-2 function transiently in the formation of mesodermal and mesenchymal derivatives. MOX-1 and MOX-2 are implicated in the early steps of mesoderm formation during gastrulation. In addition, the MOX proteins are also involved in somatic differentiation. Significantly, MOX-1 associates more strongly with Pax1, whereas MOX-2 preferentially associates with Pax3. Specifically, expression of MOX-2, also known as Mesenchyme homeobox 2 and GAX, has been shown to be critical in axial skeleton development. MOX-2 is not needed for the migration of myogenic precursors into the limb bud, but it is essential for normal appendicular muscle formation and for the normal regulation of myogenic genes. MOX-2 is expressed in placental tissue. The human MEOX2 gene maps to chromosome 7p22.1-p21.3 and encodes the MOX-2 protein. Mutations in the gene may be involved in craniofacial and/or skeletal abnormalities.
General readings
Candia A F., et al. (1996) Int J Dev Biol. 40:1179-1184Mankoo B S., et al. (1999) Nature. 400:69-73.
Immunoperoxidase of monoclonal antibody to MEOX2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1 ug/ml]
Immunofluorescence of monoclonal antibody to MEOX2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of MEOX2 expression in transfected 293T cell line by MEOX2 monoclonal antibody (M03), clone 6A5.Lane 1: MEOX2 transfected lysate(33 KDa).Lane 2: Non-transfected lysate.
MEOX2 monoclonal antibody (M03), clone 6A5 Western Blot analysis of MEOX2 expression in HeLa ( Cat # L013V1 ).
MEOX2 monoclonal antibody (M03), clone 6A5. Western Blot analysis of MEOX2 expression in NIH/3T3 ( Cat # L018V1 ).
MEOX2 monoclonal antibody (M03), clone 6A5. Western Blot analysis of MEOX2 expression in PC-12 ( Cat # L012V1 ).
MEOX2 monoclonal antibody (M03), clone 6A5. Western Blot analysis of MEOX2 expression in Hela NE ( Cat # L013V3 ).
MEOX2 monoclonal antibody (M03), clone 6A5. Western Blot analysis of MEOX2 expression in Raw 264.7 ( Cat # L024V1 ).
Detection limit for recombinant GST tagged MEOX2 is approximately 0.3ng/ml as a capture antibody.
Western Blot analysis of MEOX2 expression in transfected 293T cell line by MEOX2 monoclonal antibody (M10), clone 4E10.Lane 1: MEOX2 transfected lysate(34.845 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged MEOX2 is approximately 1ng/ml as a capture antibody.
MEOX2 monoclonal antibody (M11), clone 4B2 Western Blot analysis of MEOX2 expression in MCF-7 ( Cat # L046V1 ).
Detection limit for recombinant GST tagged MEOX2 is approximately 3ng/ml as a capture antibody.
Human Jurkat; WB Suggested Anti-MEOX2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; MEOX2 antibody - C-terminal region (ARP38701_T100) in Human Jurkat cells using Western Blot
Transfected 293T; WB Suggested Anti-MEOX2 Antibody Titration: 2.5ug/ml. Positive Control: Transfected 293T; MEOX2 antibody - N-terminal region (ARP32698_T100) in Transfected 293T cells using Western Blot
Human Jurkat; WB Suggested Anti-MEOX2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; MEOX2 antibody - N-terminal region (ARP38700_T100) in Human Jurkat cells using Western Blot
Immunofluorescence of monoclonal antibody to MEOX2 on HeLa cell. [antibody concentration 10 ug/ml]
MEOX2 monoclonal antibody (M02), clone 5A6. Western Blot analysis of MEOX2 expression in NIH/3T3 ( Cat # L018V1 ).
MEOX2 monoclonal antibody (M02), clone 5A6. Western Blot analysis of MEOX2 expression in Raw 264.7 ( Cat # L024V1 ).
MEOX2 monoclonal antibody (M02), clone 5A6 Western Blot analysis of MEOX2 expression in HeLa ( Cat # L013V1 ).
MEOX2 monoclonal antibody (M02), clone 5A6. Western Blot analysis of MEOX2 expression in PC-12 ( Cat # L012V1 ).
Detection limit for recombinant GST tagged MEOX2 is approximately 3ng/ml as a capture antibody.
Western blot(WB) analysis of MOX-2 antibody (Cat.-No.: AP06700PU-N) in extracts from COLO205 cells.
MEOX2 monoclonal antibody (M08), clone 5E1. Western Blot analysis of MEOX2 expression in NIH/3T3 ( Cat # L018V1 ).
MEOX2 monoclonal antibody (M08), clone 5E1 Western Blot analysis of MEOX2 expression in HeLa ( Cat # L013V1 ).
MEOX2 monoclonal antibody (M08), clone 5E1. Western Blot analysis of MEOX2 expression in Raw 264.7 ( Cat # L024V1 ).
MEOX2 monoclonal antibody (M08), clone 5E1. Western Blot analysis of MEOX2 expression in PC-12 ( Cat # L012V1 ).
Detection limit for recombinant GST tagged MEOX2 is approximately 3ng/ml as a capture antibody.
Western Blot analysis of MEOX2 expression in transfected 293T cell line (H00004223-T01) by MEOX2 MaxPab polyclonal antibody.Lane 1: MEOX2 transfected lysate(33.50 KDa).Lane 2: Non-transfected lysate.
MEOX2 MaxPab rabbit polyclonal antibody. Western Blot analysis of MEOX2 expression in PC-12.
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to MEOX2 on HeLa cell. [antibody concentration 10 µg/ml]
MEOX2 monoclonal antibody clone 6A5. Western Blot analysis of MEOX2 expression in NIH/3T3.
MEOX2 monoclonal antibody clone 6A5. Western Blot analysis of MEOX2 expression in PC-12.
MEOX2 monoclonal antibody clone 6A5 Western Blot analysis of MEOX2 expression in HeLa.
Detection limit for recombinant GST tagged MEOX2 is approximately 0.3ng/ml as a capture antibody.
Immunohistochemical analysis of MEOX2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of MEOX2 expression in HeLa (A) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEOX2 (RC201948, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEOX2.
Western blot analysis of extracts from COLO205 cells, using MEOX2 antibody.The lane on the right is treated with the synthesized peptide.
MEOX2 Antibody (Center) (Cat. #TA324647) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the MEOX2 Antibody detected the MEOX2 protein (arrow).
IF image of NIH-3T3 cell stained with MEOX2 Antibody (Center)(Cat#TA324647).NIH-3T3 cells were incubated with MEOX2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).MEOX2 immunoreactivity is localized to Nucleus significantly.
WB Suggested Anti-MEOX2 Antibody Titration: 2.5ug/ml; Positive Control: Transfected 293T
WB Suggested Anti-MEOX2 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate
WB Suggested Anti-MEOX2 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314189 | MEOX2 antibody |
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| Rabbit polyclonal anti-MEOX2 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA324647 | MEOX2 antibody |
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| Rabbit polyclonal MEOX2 Antibody (Center) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.4 ml /
$415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AP06700PU-N | MEOX2 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
$370.00
|
||
| OriGene Technologies GmbH | |||||||