MCAT antibody
Principal name
MCAT antibody
Alternative names for MCAT antibody
Mitochondrial malonyltransferase, MCT, Malonyl CoA-acyl carrier protein transacylase, mitochondrial
Ncbi ID
9606, NP_055322, NP_055322.1, NM_173467, NM_014507, NP_775738, NP_001025185.1, NM_001030014
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of MCAT antibody
- Kit Component:
- - KN309860G1, Mcat gRNA vector 1 in pCas-Guide vector
- KN309860G2, Mcat gRNA vector 2 in pCas-Guide vector
- KN309860D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to MT on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
MT monoclonal antibody (M01), clone 2F2 Western Blot analysis of MT expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged MT is approximately 1ng/ml as a capture antibody.
Western Blot analysis of MCAT expression in transfected 293T cell line (H00027349-T01) by MCAT MaxPab polyclonal antibody.Lane1:MT transfected lysate(19.8 KDa).Lane2:Non-transfected lysate.
Western Blot analysis of MCAT expression in transfected 293T cell line (H00027349-T02) by MCAT MaxPab polyclonal antibody.Lane 1: MCAT transfected lysate(19.20 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of MCAT transfected lysate using anti-MCAT MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MCAT MaxPab mouse polyclonal antibody (B01) (H00027349-B01).
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCAT(RC207943) using Anti-MCAT mouse monoclonal antibody (TA507070) at 1:100 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCAT (RC207943, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCAT.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MCAT monoclonal antibody at 1:400 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCAT(RC207943) using Anti-MCAT mouse monoclonal antibody (TA507071) at 1:100 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCAT (RC207943, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCAT.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MCAT monoclonal antibody at 1:400 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCAT (RC207943, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCAT.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA507070 | MCAT antibody |
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| MCAT mouse monoclonal antibody, clone OTI2B7 (formerly 2B7) | |||||||
 |
Mouse | IgG2a | OTI2B7 | Purified | Hu | ICC/IF, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|
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| TA507071 | MCAT antibody |
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| MCAT mouse monoclonal antibody, clone OTI2G1 (formerly 2G1) | |||||||
 |
Mouse | IgG2a | OTI2G1 | Purified | Hu | ICC/IF, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA507080 | MCAT antibody |
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| MCAT mouse monoclonal antibody, clone OTI2H4 (formerly 2H4) | |||||||
 |
Mouse | IgG2a | OTI2H4 | Purified | Hu | WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307943 | MCAT (transcript variant 1) |
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| Recombinant protein of human malonyl CoA:ACP acyltransferase (mitochondrial) (MCAT), nuclear gene encoding mitochondrial protein, transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY406620 | MCAT overexpression lysate |
|
 |
0.1 mg /
$295.00
|
|
| OriGene Technologies, Inc. | ||
| LY415211 | MCAT overexpression lysate |
|
|
0.1 mg /
$295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205958L2V | Lenti ORF particles, Mcat (GFP-tagged) - Mouse malonyl CoA:ACP acyltransferase (mitochondrial) (Mcat), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
$930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205958L1V | Lenti ORF particles, Mcat (Myc-DDK-tagged) - Mouse malonyl CoA:ACP acyltransferase (mitochondrial) (Mcat), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
$830.00
|
|
| OriGene Technologies, Inc. | ||