MAST4 antibody
Principal name
MAST4 antibody
Alternative names for MAST4 antibody
Microtubule-associated serine/threonine-protein kinase 4, LOC375449
Gene ID
375449 (MAST4)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)
Background of MAST4 antibody
- Kit Component:
- - KN207706G1, MAST4 gRNA vector 1 in pCas-Guide vector
- KN207706G2, MAST4 gRNA vector 2 in pCas-Guide vector
- KN207706D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of MAST4 expression in transfected 293T cell line (H00375449-T01) by MAST4 MaxPab polyclonal antibody.Lane 1: LOC375449 transfected lysate(27.5 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MAST4 expression in transfected 293T cell line (H00375449-T01) by MAST4 MaxPab polyclonal antibody.Lane 1: LOC375449 transfected lysate(27.5 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical analysis of MAST4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of MAST4 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of MAST4 expression in HeLa (A); HuvEc (B) whole cell lysates.
Western blot analysis of extracts from Jurkat cells, COLO cells, HUVEC cells and MCF-7 cells, using MAST4 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human testis tissue using MAST4 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of COS-7 cells, using MAST4 antibody.The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA316393 | MAST4 antibody |
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| Rabbit polyclonal anti-MAST4 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P, WB |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA347763 | MAST4 antibody |
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| Rabbit Polyclonal Anti-MAST4 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307706 | MAST4 (transcript variant 2) |
||||
| Recombinant protein of human microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY404775 | MAST4 overexpression lysate |
|
|
0.1 mg /
$295.00
|
|
| OriGene Technologies, Inc. |