LAPTM5 antibody
Principal name
LAPTM5 antibody
Alternative names for LAPTM5 antibody
LAPTM5, KIAA0085, Retinoic acid-inducible E3 protein, Lysosomal-associated transmembrane protein 5, Lysosomal-associated multitransmembrane protein 5
Ncbi ID
9606, NP_006753, NP_006753.1, NP_034816.1, BC020993, NM_010686, NM_006762
Available reactivities
Available hosts
Available applications
Flow Cytometry (F), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)
Background of LAPTM5 antibody
- Kit Component:
- - KN309133G1, Laptm5 gRNA vector 1 in pCas-Guide vector
- KN309133G2, Laptm5 gRNA vector 2 in pCas-Guide vector
- KN309133D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunohistochemistry analysis in human lymph node (formalin-fixed, paraffin-embedded) using LAPTM5 antibody Cat.-No. AP52440PU-N (N-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LAPTM5 antibody for IHC. Clinical relevance has not been evaluated.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane) using LAPTM5 antibody Cat.-No. AP52440PU-N (N-term). This demonstrates the LAPTM5 antibody detected the LAPTM5 protein (arrow).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram) using LAPTM5 antibody Cat.-No. AP52440PU-N (N-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAPTM5 (RC217813, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAPTM5.
ELISA: LAPTM5 Antibody
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP317813 | LAPTM5 |
||||
| Recombinant protein of human lysosomal multispanning membrane protein 5 (LAPTM5) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
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| OriGene Technologies, Inc. | |||||