KLF9 antibody
Principal name
KLF9 antibody
Alternative names for KLF9 antibody
KLF9, BTEB, BTEB1, Krueppel-like factor 9, Transcription factor BTEB1, BTE-binding protein 1, GC-box-binding protein 1, Basic transcription element-binding protein 1
Ncbi ID
9606, NP_001197, ABZ92418.1, NP_001197.1, NM_057211, NM_001206
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of KLF9 antibody
KLF9 is a transcription factor that binds to GC box elements located in the promoter. Binding of the encoded protein to a single GC box inhibits mRNA expression while binding to tandemly repeated GC box elements activates transcription.The protein encoded by this gene is a transcription factor that binds to GC box elements located in the promoter. Binding of the encoded protein to a single GC box inhibits mRNA expression while binding to tandemly repeated GC box elements activates transcription. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Imataka,H., et al., (1994) J. Biol. Chem. 269 (32), 20668-20673
Detection limit for recombinant GST tagged KLF9 is approximately 0.03ng/ml as a capture antibody.
Detection limit for recombinant GST tagged KLF9 is approximately 0.03ng/ml as a capture antibody.
Human Testis; Testis; KLF9 Antibody - N-terminal region (ARP31369_P050) in Human Testis cells using Immunohistochemistry
Human kidney; Rabbit Anti-KL9 Antibody. Catalog Number: ARP31369. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; KLF9 Antibody - N-terminal region (ARP31369_P050) in Human kidney cells using Immunohistochemistry
Human Fetal Liver; Host: Rabbit. Target Name: KLF9. Sample Tissue: Fetal Liver lysates. Antibody Dilution: 1.0ug/ml.; KLF9 Antibody - N-terminal region (ARP31369_P050) in Human Fetal Liver cells using Western Blot
Human Jurkat; WB Suggested Anti-KLF9 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Jurkat; KLF9 Antibody - N-terminal region (ARP31369_P050) in Human Jurkat cells using Western Blot
Human 721_B; WB Suggested Anti-KLF9 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. There is BioGPS gene expression data showing that KLF9 is expressed in 721_B.; KLF9 antibody - N-terminal region (ARP58083_P050) in Human 721_B cells using Western Blot
Human Testis; Testis; KLF9 Antibody - N-terminal region (AP42022PU-N) in Human Testis cells using Immunohistochemistry
Human kidney; Rabbit Anti-KL9 Antibody. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; KLF9 Antibody - N-terminal region (AP42022PU-N) in Human kidney cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-KLF9 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Jurkat; KLF9 Antibody - N-terminal region (AP42022PU-N) in Human Jurkat cells using Western Blot
Human Fetal Liver; Host: Rabbit. Target Name: KLF9. Sample Tissue: Fetal Liver lysates. Antibody Dilution: 1.0ug/ml.; KLF9 Antibody - N-terminal region (AP42022PU-N) in Human Fetal Liver cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLF9 (RC210147, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLF9.(1:2000)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-KLF9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808444)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KLF9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808444)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KLF9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808444)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KLF9 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808444)(1:150)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-KLF9 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808444)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KLF9 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808444)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-KLF9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808444)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-KLF9 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808444)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-KLF9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808444)(1:150)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-KLF9 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808444)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KLF9 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808444)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KLF9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808444)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KLF9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808444)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLF9 (RC210147, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLF9.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLF9 (RC210147, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLF9.(1:2000)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KLF9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808446)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KLF9 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808446)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KLF9 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808446)(1:150)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-KLF9 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808446)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KLF9 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808446)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLF9 (RC210147, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLF9.(1:2000)
KLF9 Antibody (N-term) (Cat. #TA324593) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the KLF9 antibody detected the KLF9 protein (arrow).
IF image of U251 cell stained with KLF9 Antibody (N-term)(Cat#TA324593).U251 cells were incubated with KLF9 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . KLF9 immunoreactivity is localized to vesicles and Nucleus significantly.
WB Suggested Anti-KLF9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 721_B cell lysateThere is BioGPS gene expression data showing that KLF9 is expressed in 721_B
Host: Rabbit; Target Name: KLF9; Sample Tissue: Fetal Liver lysates; Antibody Dilution: 1.0ug/ml
WB Suggested Anti-KLF9 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positive Control: Jurkat
Rabbit Anti-KL9 Antibody; Paraffin Embedded Tissue: Human Kidney; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Testis
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760077 | KLF9 (full length, N-term HIS tag) |
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| Recombinant protein ofhuman Kruppel-like factor 9 (KLF9), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
$205.00
|
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| OriGene Technologies, Inc. | |||||