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KCNN4 antibody

Principal name

KCNN4 antibody

Alternative names for KCNN4 antibody

KCNN4, IK1, IKCA1, KCA4, SK4, KCa3.1, Putative Gardos channel, Intermediate conductance calcium-activated potassium channel protein 4

SwissProt ID

O15554 (Human), O89109 (Mouse), Q9QYW1 (Rat)

Gene ID

3783 (KCNN4), 65206 (Kcnn4), 16534

Ncbi ID

NP_002241.1, NP_002241, AAH15337, NM_002250, NP_075410.1, NM_008433, NM_001163510, NM_023021

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Bt (Bat), Can (Canine), GP (Guinea Pig), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Bear, Eq (Equine)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E)

Background of KCNN4 antibody

KCNN4 is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. KCNN4 may be part of the predominant calcium-activated potassium channel in T-lymphocytes. The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human KCNN4 with an internal ID of P06329

General readings

Gao,Y., (2008) J. Biol. Chem. 283 (14), 9049-9059

Human Hela; Host: Rabbit. Target Name: KCNN4. Sample Tissue: Hela. Antibody Dilution: 1.0ug/ml.; KCNN4 antibody - C-terminal region (ARP35098_P050) in Human Hela cells using Western Blot

ARP35098_P050

Human HepG2; Host: Rabbit. Target Name: KCNN4. Sample Tissue: HepG2. Antibody Dilution: 1.0ug/ml.; KCNN4 antibody - C-terminal region (ARP35098_P050) in Human HepG2 cells using Western Blot

ARP35098_P050

Human Jurkat; Host: Rabbit. Target Name: KCNN4. Sample Tissue: Jurkat. Antibody Dilution: 1.0ug/ml.; KCNN4 antibody - C-terminal region (ARP35098_P050) in Human Jurkat cells using Western Blot

ARP35098_P050

Human MCF7; Host: Rabbit. Target Name: KCNN4. Sample Tissue: MCF7. Antibody Dilution: 1.0ug/ml.; KCNN4 antibody - C-terminal region (ARP35098_P050) in Human MCF7 cells using Western Blot

ARP35098_P050

Human HepG2; WB Suggested Anti-KCNN4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; KCNN4 antibody - C-terminal region (ARP35098_P050) in Human HepG2 cells using Western Blot

ARP35098_P050

KCNN4 (KCA3.1) Polyclonal Antibody

AP23278PU-N

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP31012PU-N

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP31012PU-N

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP31012PU-N

Western blot analysis of HEK-293-KCa3.1 (1, 2) and K562 (3, 4) cells: 1, 3. Anti-KCa3.1 (SK4, IKCa1) antibody , (1:200). 2, 4. Anti-KCa3.1 (SK4, IKCa1) antibody, preincubated with the control peptide antigen.

TA328961

Western blot analysis of extracts of various cell lines, using KCNN4 antibody

TA332480

Immunofluorescence analysis of HeLa cell using KCNN4 antibody. Blue: DAPI for nuclear staining

TA332480

WB Suggested Anti-KCNN4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate

TA338529

Host: Rabbit; Target Name: KCNN4; Sample Tissue: Hela; Antibody Dilution: 1.0 ug/ml

TA338529

Host: Rabbit; Target Name: KCNN4; Sample Tissue: MCF7; Antibody Dilution: 1.0 ug/ml

TA338529

Host: Rabbit; Target Name: KCNN4; Sample Tissue: Jurkat; Antibody Dilution: 1.0 ug/ml

TA338529

Host: Rabbit; Target Name: KCNN4; Sample Tissue: HepG2; Antibody Dilution: 1.0 ug/ml

TA338529

Anti-KCNN4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA341228

Anti-KCNN4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA341229

Western blot analysis of KCNN4 expression in K562 (A); HepG2 (B); mouse brain (C); rat brain (D) whole cell lysates.

Immunohistochemical analysis of KCNN4 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: NIH/3T3 cells, Primary antibody: (KCNN4 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds

TA351323

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (KCNN4 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)

TA351323

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (KCNN4 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)

TA351323

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
AP31012PU-N	KCNN4 (227-239) antibody
	Goat	IgG	Aff - Purified	Bov, Can, Elephant, Hu, Rb	P	50 µg / $425.00
						OriGene Technologies GmbH
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