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IRAK4 antibody

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Principal name

IRAK4 antibody

Alternative names for IRAK4 antibody

IRAK-4, NY-REN-64, Interleukin-1 receptor-associated kinase-like

SwissProt ID

Q1RMT8 (Bovin), Q8R4K2 (Mouse), Q9NWZ3 (Human)

Gene ID

51135 (IRAK4), 266632 (Irak4)

Ncbi ID

NP_001107654.1, 9606, NP_001107654, AAX22228.1, NP_057207, AAM15772, NP_084202.2, NM_029926, NM_016123, NM_001145257

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Bt (Bat), Can (Canine), Eq (Equine), Hst (Hamster), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Sh (Sheep), L (Lizard), Bear, Rt (Rat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Dot blot (Dot), Immunoprecipitation (IP), Flow Cytometry (F), ELISA (detection) (E(detection))

Background of IRAK4 antibody

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. IL-1 receptor-associated kinases (IRAKs) are important mediators in the signal transduction of Toll/IL-1 receptor (TIR) family members. The cytoplasmic domains of TIR proteins interact with the adapter protein, MyD88. MyD88 then recruits IRAKs (IRAK-1, -2, and M), which in turn interact with other adapter molecules, such as TRAF6 to activate NF-kB and MAPK pathways. Recently, a new member of this family, IRAK-4 has been identified. IRAK-4 may act as an upstream activator of IRAK-1. IRAK-4 is important for LPS activation of TLRS. Mice lacking IRAK-4 are resistant to lethal doses of LPS and are also severely impaired in their responses to viral and bacterial challenges.

General readings

Silva, L.K., et al. Eur. J. Hum. Genet. 18(11):1221-1227(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
McDonald, D.R., et al. J. Allergy Clin. Immunol. 126(2):332-337(2010)
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Wang, Z., et al. Structure 14(12):1835-1844(2006)

AP00252PU-N - IRAK4
Western blot analysis of IRAK-4 expression with HeLa cell lysate.
H00051135-M03 - IRAK4
Detection limit for recombinant GST tagged IRAK4 is approximately 0.03ng/ml as a capture antibody.
H00051135-M04 - IRAK4
Immunoperoxidase of monoclonal antibody to IRAK4 on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml]
H00051135-M04 - IRAK4
IRAK4 monoclonal antibody (M04), clone 2D3 Western Blot analysis of IRAK4 expression in K-562 ( Cat # L009V1 ).
H00051135-M04 - IRAK4
Detection limit for recombinant GST tagged IRAK4 is approximately 1ng/ml as a capture antibody.
H00051135-M05 - IRAK4
IRAK4 monoclonal antibody (M05), clone 1D4 Western Blot analysis of IRAK4 expression in K-562 ( Cat # L009V1 ).
H00051135-M05 - IRAK4
Detection limit for recombinant GST tagged IRAK4 is approximately 0.1ng/ml as a capture antibody.
H00051135-M11 - IRAK4
Detection limit for recombinant GST tagged IRAK4 is approximately 1ng/ml as a capture antibody.
H00051135-M09 - IRAK4
Detection limit for recombinant GST tagged IRAK4 is approximately 0.3ng/ml as a capture antibody.
H00051135-M06 - IRAK4
Detection limit for recombinant GST tagged IRAK4 is approximately 1ng/ml as a capture antibody.
H00051135-M08 - IRAK4
Detection limit for recombinant GST tagged IRAK4 is approximately 0.3ng/ml as a capture antibody.
H00051135-M07 - IRAK4
Detection limit for recombinant GST tagged IRAK4 is approximately 1ng/ml as a capture antibody.
AP07319PU-N - IRAK4
Spleen: Formalin-Fixed Paraffin-Embedded (FFPE)
PAB6731 - IRAK4
IRAK4 polyclonal antibody ( Cat # PAB6731 ) ( 1 µg/ml ) staining of Hela lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
AP08454PU-N - IRAK4
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
MAB2538 - IRAK4
The 5 x 107 Jurkat cells were stimulated by 50 ng/ml IL-1 for 10 min, collected, lysed, resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with IRAK4 ( phospho T345 ) monoclonal antibody, clone A8A8 ( Cat # MAB2538 ) at 1 : 500 ( lane 1 ).Lane 1 was pre-incubated with Phosphospecific peptide.Lane 2 was pre-incubate with non-phospho specific peptide.An immunoreactive band is observed around ~51 kDa.
MAB2538 - IRAK4
Dot Blot : 1 µg peptide was blot onto NC membrane.A : IRAK4 ( pT345 ). B : IRAK4 ( non phosphorylated ). Followed by IRAK4 ( phospho T345 ) monoclonal antibody, clone A8A8 ( Cat # MAB2538 ) incubation at a 1 : 1000 dilution.
PAB10251 - IRAK4
Immunocytochemistry using IRAK4 polyclonal antibody ( Cat # PAB10251 ) shows staining of IRAK4 in HeLa cells.The cells were fixed with paraformaldehyde and reacted with the primary antibody diluted to 1 : 100.
PAB10251 - IRAK4
Western blot using IRAK4 polyclonal antibody ( Cat # PAB10251 ) shows detection of IRAK4 in HeLa whole cell lysate. The membrane was probed with the primary antibody dilutedto 1:1000 ( lane 1 ), 1 : 500 ( lane 2 ), and 1 : 250 ( lane 3 ) respectively.
PAB8788 - IRAK4
Western blot analysis of IRAK4 expression HeLa cell lysate.
AP23599PU-N - IRAK4
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP23599PU-N - IRAK4
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP23599PU-N - IRAK4
(1µg/ml) Staining of Hela lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
DDX0340A488-50 - IRAK4
IHC staining of human FFPE tonsil section (Vermi William courtesy)
DDX0340A488-50 - IRAK4
IF staining of human tonsil with 6F8 (DDX0340)
DDX0340A488-50 - IRAK4
WB with 6F8 (Innateplateform, CLARA, Lyon)
DDX0340A488-50 - IRAK4
FACS staining of caninecell line with 6F8 (BonnefontC, personalcomm., 2011)
DDX0341A488-50 - IRAK4
DDX0341A488-50 - IRAK4
AP52231PU-N - IRAK4
Western blot analysis of IRAK4 Antibody (N-term) Cat.-No AP52231PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the IRAK4 antibody detected the IRAK4 protein (arrow).
AM06659SU-N - IRAK4
Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues using IRAK4 mouse mAb with DAB staining.
AM06659SU-N - IRAK4
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using IRAK4 mouse mAb with DAB staining.
AM06659SU-N - IRAK4
Western blot analysis using IRAK4 mouse mAb against THP-1 (1), Hela (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.
AM06659SU-N - IRAK4
Western blot analysis using IRAK4 mAb against human IRAK4 (AA: 21-198) recombinant protein. (Expected MW is 45.4 kDa)
AM06659SU-N - IRAK4
Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)
AM06659SU-N - IRAK4
Flow cytometric analysis of Hela cells using IRAK4 mouse mAb (blue) and negative control (red).
TP303041 - IRAK4
TA302664 - IRAK4
TA302664 (1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA306123 - IRAK4
Western blot analysis of IRAK-4 in HeLa cell lysate with IRAK-4 antibody at 1 (lane 1), 2 (lane 2), and 4 (lane 3) ug/mL, respectively.
TA306123 - IRAK4
Immunocytochemistry of IRAK-4 in K562 cells with IRAK-4 antibody at 10 ug/mL.
TA306123 - IRAK4
Immunofluorescence of IRAK-4 in K562 cells with IRAK-4 antibody at 10 ug/mL.

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IRAK4 (N-term) antibody
  Goat Polyclonal Antibody against IRAK4 (N Terminus)  
TA302664 (1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, WB
0.1 mg / $360.00
  OriGene Technologies, Inc.

IRAK4 antibody
  Rabbit Polyclonal IRAK-4 Antibody  
Western blot analysis of IRAK-4 in HeLa cell lysate with IRAK-4 antibody at 1 (lane 1), 2 (lane 2), and 4 (lane 3) ug/mL, respectively. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / $415.00
  OriGene Technologies, Inc.
+2 additional images

IRAK4 antibody
Western blot analysis of IRAK-4 expression with HeLa cell lysate. Rabbit Aff - Purified Hu WB
0.1 mg / $380.00
  OriGene Technologies GmbH

IRAK4 antibody
Spleen: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µg / $425.00
  OriGene Technologies GmbH

IRAK4 (38-54, 120-136) antibody
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µg / $425.00
  OriGene Technologies GmbH

IRAK4 (2-13) antibody
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bt, Elephant, Eq, Hu, Mky E, P, WB
50 µg / $425.00
  OriGene Technologies GmbH
+2 additional images

IRAK4 (N-term) antibody
Western blot analysis of IRAK4 Antibody (N-term) Cat.-No AP52231PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the IRAK4 antibody detected the IRAK4 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / $415.00
  OriGene Technologies GmbH

7 Item(s)

per page

Active Filters

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Product Type
  1. Primary Antibody (7)
Target
  1. IRAK4 (7)
Property
  1. 2-13 (1)
  2. 38-54, 120-136 (1)
  3. N-term (2)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (2)
  3. Bat (Bt) (1)
  4. Elephant (1)
  5. Equine (Eq) (1)
  6. Monkey (Mky) (1)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Enzyme Immunoassay (E) (3)
  3. Paraffin Sections (P) (3)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
Presentation
  1. Aff - Purified (7)
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  1. Rabbit (5)
  2. Goat (2)
Images
  1. 1 image (7)
  2. 3+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (3)
  3. Immunofluorescence (IF) (1)
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  1. OriGene Technologies (7)
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