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HMGB4 antibody

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Principal name

HMGB4 antibody

Alternative names for HMGB4 antibody

High mobility group protein B4

SwissProt ID

Q32L34 (Bovin), Q6P8W9 (Mouse), Q8WW32 (Human)

Gene ID

127540 (HMGB4), 69317 (Hmgb4)

Ncbi ID

NP_660206, 9606, NP_660206.2, NM_145205, NP_081312.2, NM_027036, NM_001008728

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of HMGB4 antibody

High mobility group protein B4?is a?transcription factor?that in humans is encoded by the?HMGB4?gene.HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain; but not in many other tissues. HMGB4 associates with chromatin; and in transfection assays; in contrast to HMGB1; it acts as a potent transcriptiol repressor. During spermatogenesis; HMGB4 is present in the euchromatin of late pachytene spermatocytes and haploid round spermatids; whereas stronger expression is observed during the elongation phase; where it localizes to the basal pole of the nucleus in a manner mutually exclusive with H1FNT (H1T2) localized at the apical pole. HMGB4 basal localization is lost in H1FNT-mutant spermatids; showing that H1FNT provides a positiol cue for organizing chromatin domains within the nucleus. These results show that HMGB4 and H1FNT specify distinct chromatin domains at the apical and basal poles of the elongating spermatid nucleus.

General readings

Gerhard,D.S., Genome Res. 14 (10B), 2121-2127 (2004)

ARP39852_P050 - HMGB4
Human Jurkat; WB Suggested Anti-HMGB4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; HMGB4 antibody - C-terminal region (ARP39852_P050) in Human Jurkat cells using Western Blot
PAB7982 - HMGB4
Western blot analysis of rat testis tissue lysate. Using HMGB4 polyclonal antibody ( Cat # PAB7982 ) .
ARP39851_P050 - HMGB4
Human HT1080; WB Suggested Anti-HMGB4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HT1080 cell lysate; HMGB4 antibody - N-terminal region (ARP39851_P050) in Human HT1080 cells using Western Blot
AP23252PU-N - HMGB4
Western blot analysis of rat testicle tissue lysis using HMGB4 antibody
TA808611 - HMGB4
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HMGB4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808611)(1:150)
TA808611 - HMGB4
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HMGB4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808611)(1:150)
TA808611 - HMGB4
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HMGB4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808611)(1:150)
TA808611 - HMGB4
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HMGB4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808611)(1:150)
TA808611 - HMGB4
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HMGB4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808611)(1:150)
TA808611 - HMGB4
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMGB4 (RC209978, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMGB4.(1:2000)
TA322036 - HMGB4
Predicted band size: 22 kDa . Positive control: 231 cell and human fetal liver tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: 231 cell lysate Lane2: Human fetal liver tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
TA345518 - HMGB4
WB Suggested Anti-HMGB4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HT1080 cell lysate
TA345519 - HMGB4
WB Suggested Anti-HMGB4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate

6 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HMGB4 antibody
  Anti-HMGB4 Rabbit Polyclonal Antibody  
Predicted band size: 22 kDa . Positive control: 231 cell and human fetal liver tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: 231 cell lysate Lane2: Human fetal liver tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / $335.00
  OriGene Technologies, Inc.

HMGB4 (N-term) antibody
Western blot analysis of rat testicle tissue lysis using HMGB4 antibody Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / $390.00
  OriGene Technologies GmbH

Lysates

Catalog No.    

HMGB4 overexpression lysate
HMGB4 overexpression lysate  
0.1 mg / $295.00
  OriGene Technologies, Inc.

HMGB4 overexpression lysate
HMGB4 overexpression lysate  
0.1 mg / $315.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Hmgb4 (GFP-tagged) - Mouse high-mobility group box 4 (Hmgb4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Hmgb4 (GFP-tagged) - Mouse high-mobility group box 4 (Hmgb4), 200 uL, >10^7 TU/mL  
200 µl / $930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Hmgb4 (Myc-DDK-tagged) - Mouse high-mobility group box 4 (Hmgb4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Hmgb4 (Myc-DDK-tagged) - Mouse high-mobility group box 4 (Hmgb4), 200 uL, >10^7 TU/mL  
200 µl / $830.00
  OriGene Technologies, Inc.
 

6 Item(s)

per page

Available Filters

Product Type
  1. Lysate (2)
  2. Mouse Lenti ORF Particle (2)
  3. Primary Antibody (2)
Clonality
  1. Polyclonal (2)
Target
  1. HMGB4 (2)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (2)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Enzyme Immunoassay (E) (1)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Aff - Purified (1)
  2. Purified (1)
Host
  1. Rabbit (2)
Images
  1. 1 image (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
Manufacturer
  1. OriGene Technologies (6)
Service
 
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