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Histone H2B antibody

Principal name

Histone H2B antibody

Alternative names for Histone H2B antibody

H2B Histone Family, HIST1H2B

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mam (Mammalia), Arabidopsis, Can (Canine), Chimp (Chimpanzee), Chk (Chicken), Dros (Drosophila), Ze (Zebrafish), Hst (Hamster)

Available hosts

Rabbit, Sheep, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), ChIP assay (ChIP)

Background of Histone H2B antibody

In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers.

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3 and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. Whilst the core histones are highly conserved across a wide range of organisms, the linker histones are less conserved.

General readings

1. Braunstein M, et al. Mol. Cell. Biol. 16:4349–56, 1996.
2. Workman JL, Kingston RE,. Annu. Rev. Biochem. 67:
545–579, 1998.
3. Cheung, P. et al. Cell103, 263–27, 2000.
4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005.
5. Ahn SH, et al. Cell Cycle 4:780-783, 2005.

Western blot analysis of Histone H2B expression with Jurkat cell lysate.

AP00282PU-N

Immunohistochemistry: Histone H2B Antibody (EP957Y) [NB110-57038] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Histone H2B (NB110-57038)

NB110-57038

Immunofluorescent: Histone H2B Antibody (EP957Y) [NB110-57038] - Fluorescent staining of HeLa cells using anti-Histone H2B (NB110-57038).

NB110-57038

Western Blot: Histone H2B Antibody (EP957Y) [NB110-57038] - Western blot analysis on A431 cell lysate using anti-Histone H2B (NB110-57038), 1:20,000 dilution.

NB110-57038

Western blot analysis of Hela cell lysate using 1 µg/ml Rabbit Anti-Histone 2B Polyclonal Antibody (GTX21790). The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

GTX21790

Immunohistochemistry analysis of Histone H2B Antibody (Cat.-No AP01181PU-N) in paraffin-embedded human brain tissue.

AP01181PU-N

Western blot (WB) analysis of Histone H2B Antibody (Cat.-No AP01181PU-N) in extracts from A549 cells.

AP01181PU-N

Heart: Formalin-Fixed Paraffin-Embedded (FFPE)

AP08442PU-N

Western blot of fractionated Cos-7 cells using antibody anti-Histone H2B.? Cos-7 cells were fractionated into nuclear & cytosolic/membrane fractions. Approximately ~15 ug of each fraction was separated on SDS PAGE. Lane 1, Nuclear fraction.

AP08442PU-N

Western blot of Histone H2B in human PBMC lysate with antibody

AP08442PU-N

Western blot of Histone H2B using antibody at 1/250 in nuclear and cytoplasmic fractionsumade from Drosophila head extracts.

AP08442PU-N

AP08814SU-N staining Histone H2B in Formalin-Fixed, Paraffin-Embedded Human Breast tissue by Immunohistochemistry.

AP08814SU-N

Immunohistochemistry: Histone H2B Antibody [NBP1-19400] - Histone H2B Antibody [NBP1-19400] - paraffin-embedded human breast carcinoma tissue.

NBP1-19400

Western Blot: Histone H2B Antibody [NBP1-19400] - extracts from COS7 cells.

NBP1-19400

Chromatin Immunoprecipitation: Histone H2B Antibody [NBP1-19400] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NBP1-19400 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

NBP1-19400

Immunohistochemistry analyzes of Histone H2B antibody (AP20239PU-N) in paraffin-embedded human breast carcinoma tissue.

AP20239PU-N

Western blot analysis of Histone H2B antibody (AP20239PU-N) in extracts from COS7 cells.

AP20239PU-N

Western blot analysis of extracts from Jurkat cells, treated with UV 15', using Histone H2B (Ab-14) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Histone H2B Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using Histone H2B Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from A549 cells, using Histone H2B Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H2B (Acetyl-Lys12) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys12) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Histone H2B (Acetyl-Lys12) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H2B (Acetyl-Lys15) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Ab-5) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using Histone H2B (Ab-5) Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Ab-12) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using Histone H2B (Ab-12) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Histone H2B (Ab-12) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Ab-15) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H2B (Ab-15) Antibody. The lane on the right is treated with the synthesized peptide.

Immunocytochemical detection of Histone H2B on 4% PFA fixed HeLa cells with AM26683AF-N.

AM26683AF-N

Western blot analysis of Histone H2B expression in Jurkat (1), HeLa (2), Raji (3), HL-60 (4), A431 (5), L5178Y (6), WR19L (7), NIH/3T3 (8), CHO (9), PC12 (10) and Rat1 (11) using AM26683AF-N.

AM26683AF-N

Immunoprecipitation of Histone H2B from HeLa cells with mouse IgG2b (1) or AM26683AF-N (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26683AF-N.

AM26683AF-N

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
AP08442PU-N	Histone H2B (111-125) antibody
	Rabbit	IgG	Purified	Bov, Chk, Dros, Hu, Insect, Ms, Nematode, Rt, Ze	P, WB	50 µg / $425.00
						OriGene Technologies GmbH
+3 additional images	Highly validated antibody!

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