HES1 antibody
Principal name
HES1 antibody
Alternative names for HES1 antibody
HES-1, HL, HRY, Hairy-like, HHL, Hairy homolog, Hairy and enhancer of split 1
Ncbi ID
NP_005515, 9606, NP_077336.3, NP_005515.1, NP_032261.1, NM_005524, NM_008235, BC018375, NM_024360
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F), Luminex (LMNX)
Background of HES1 antibody
- Kit Component:
- - KN307671G1, Hes1 gRNA vector 1 in pCas-Guide vector
- KN307671G2, Hes1 gRNA vector 2 in pCas-Guide vector
- KN307671D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hoebeke,I., et al., (2006) Blood 107 (7), 2879-2881
HES1 monoclonal antibody (M01), clone 4D9 Western Blot analysis of HES1 expression in THP-1 ( Cat # L007V1 ).
Detection limit for recombinant GST tagged HES1 is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to HES1 on HeLa cell. [antibody concentration 10 ug/ml]
HES1 monoclonal antibody (M02), clone 3A3. Western Blot analysis of HES1 expression in H9c2(2-1).
Human Heart; . Rabbit Anti-HES1 Antibody. . Catalog Number:. ARP32372 . Paraffin Embedded Tissue: Human cardiac cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X
Human Ls174T; transfected Ls174T cell lysate at 50ug/ml.; HES1 antibody - N-terminal region (ARP32372_T100) in Human Ls174T cells using Immunofluorescence
Mouse embryonic stem; HES1 antibody - N-terminal region (ARP32372_T100) validated by WB using Mouse embryo tissue at 1:800.; HES1 antibody - N-terminal region (ARP32372_T100) in Mouse embryonic stem cells using Western Blot
Human Raji; WB Suggested Anti-HES1 Antibody Titration: 1.25ug/ml. Positive Control: Raji cell lysate. HES1 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells.; HES1 antibody - N-terminal region (ARP32372_T100) in Human Raji cells using Western Blot
Human Ls174T; HES1 antibody - N-terminal region (ARP32372_T100) validated by WB using transfected Ls174Tcell lysate at 1ug/ml.; HES1 antibody - N-terminal region (ARP32372_T100) in Human Ls174T cells using Western Blot
Human K562; K562; HES1 antibody - N-terminal region (ARP32372_T100) in Human K562 cells using Immunoprecipitation
Western blot analysis of HES1 polyclonal antibody ( Cat # PAB4860 ) in HL-60 cell lysate ( 35 µg/lane ) . HES1 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Immunoblot analysis of cell lysates. Lane 1 : BeWo cell lysate. Lane 2 : HT-29 cell lysate.
HES1 MaxPab rabbit polyclonal antibody. Western Blot analysis of HES1 expression in mouse spleen.
HES1 MaxPab rabbit polyclonal antibody. Western Blot analysis of HES1 expression in human kidney.
Western Blot analysis of HES1 expression in transfected 293T cell line (H00003280-T01) by HES1 MaxPab polyclonal antibody.Lane 1: HES1 transfected lysate(29.30 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of anti-HES1 Antibody (N-term) in HL60 cell line lysates (35ug/lane). HES1 (arrow) was detected using the purified Pab (1:60 dilution).
Rat Kidney; WB Suggested Anti-Hes1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Rat Kidney; Hes1 antibody - C-terminal region (ARP31970_P050) in Rat Kidney cells using Western Blot
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of Raji cell lysates with anti-HSE1 antibody at 1/500 dilution
Western Blot analysis using HES1 antibody Cat.-No AP55327PU-N.
HES1 measured in sporadic angiom yolipom as and normal kidney from 4 patients.
HES1 measured in sporadic angiom yolipom as and normal kidney from 4 patients.
HEK293T cells were transfected with pCMV6-ENTRY control (PS01, left panel) or HES1 cDNA (right panel) and incubated for 48 hours after transfection. The cells were detached, fixed with 2% formaldehyde for 30 min, permeabilized with 0.1% trixon-X100, and then stained with PE-labeled HES1 antibodies (TA400011).
HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY HES1 (Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1-biotin and Streptavidin-HRP.
HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY HES1 (Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1-HRP.
Immunohistochemical staining of paraffin-embedded liver within the normal limits using anti-HES1 (TA500114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-HES1 (TA500114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded breast within the normal limits using anti-HES1 (TA000114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded endometrium within the normal limits using anti-HES1 (TA500114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-HES1 (TA500114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Anti-HES1 mouse monoclonal antibody (TA500114) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HES1(RC211709).
Immunofluorescent staining of HeLa cells using anti-HES1 mouse monoclonal antibody (TA500114).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1(RC211709) (RC211709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1.
Anti-HES1 mouse monoclonal antibody (TA503958) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HES1(RC211709).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (RC211709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1.
HEK293T cells transfected with either RC211709 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HES1 antibody(TA503958), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504001)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504001)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504001)
Anti-HES1 mouse monoclonal antibody (TA504001) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HES1(RC211709).
Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-HES1 monoclonal antibody.(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (RC211709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1.
Flow cytometric Analysis of Jurkat cells, using anti-HES1 antibody(TA504001),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504002)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (RC211709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504045)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (RC211709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HES1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504046)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504046)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504046)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504046)
Anti-HES1 mouse monoclonal antibody (TA504046) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HES1(RC211709).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (RC211709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (RC211709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (RC211709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1.
HES1 ELISA with 1B5 Capture (TA600482) and 4H1 Detection (TA700482) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 ELISA with 1B5 Capture (TA600482) and 2D2 Detection (TA700483) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 Luminex ELISA with 1B5 Capture (TA600482) and 4H1 Detection (TA700482) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 Luminex ELISA with 1B5 Capture (TA600482) and 2D2 Detection (TA700483) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 ELISA with 2D2 Capture (TA600483) and 1B5 Detection (TA700484) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 Luminex ELISA with 2D2 Capture (TA600483) and 1B5 Detection (TA700484) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 ELISA with 7B9 Capture (TA600484) and 2D2 Detection (TA700483) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 ELISA with 7B9 Capture (TA600484) and 1B5 Detection (TA700484) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 Luminex ELISA with 7B9 Capture (TA600484) and 1B5 Detection (TA700484) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 Luminex ELISA with 7B9 Capture (TA600484) and 2D2 Detection (TA700483) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 ELISA with 1F11 Capture (TA600485) and 1B5 Detection (TA700484) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 Luminex ELISA with 1F11 Capture (TA600485) and 1B5 Detection (TA700484) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 ELISA with 1B5 Capture (TA600482) and 4H1 Detection (TA700482) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 Luminex ELISA with 1B5 Capture (TA600482) and 4H1 Detection (TA700482) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 ELISA with 1B5 Capture (TA600482) and 2D2 Detection (TA700483) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 Luminex ELISA with 1B5 Capture (TA600482) and 2D2 Detection (TA700483) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 Luminex ELISA with 7B9 Capture (TA600484) and 2D2 Detection (TA700483) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 ELISA with 2D2 Capture (TA600483) and 1B5 Detection (TA700484) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 ELISA with 1F11 Capture (TA600485) and 1B5 Detection (TA700484) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 Luminex ELISA with 1F11 Capture (TA600485) and 1B5 Detection (TA700484) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 Luminex ELISA with 7B9 Capture (TA600484) and 1B5 Detection (TA700484) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
HES1 Luminex ELISA with 2D2 Capture (TA600483) and 1B5 Detection (TA700484) Antibodies. Substrate used: Recombinant Human HES1 (TP311709)
Immunohistochemistry-Paraffin: Hes1 Antibody [TA336954] - Analysis of HES1 in formalin-fixed paraffin-embedded human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 1:2000.
Lysates
| Catalog No. | ||
|---|---|---|
| LY417251 | HES1 overexpression lysate |
|
 |
0.1 mg /
$295.00
|
|
| OriGene Technologies, Inc. |