GPR34 antibody
Principal name
GPR34 antibody
Alternative names for GPR34 antibody
G-protein coupled receptor 34
Ncbi ID
9606, NP_001091048.1, NP_001091048, NP_035953.3, NM_011823, NM_005300, NM_001097579
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)
Background of GPR34 antibody
- Kit Component:
- - KN307240G1, Gpr34 gRNA vector 1 in pCas-Guide vector
- KN307240G2, Gpr34 gRNA vector 2 in pCas-Guide vector
- KN307240D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Engemaier, E., et al. Genomics 87(2):254-264 (2006)
Oh, J.H., et al. Mamm. Genome 16(12):942-954 (2005)
Jacobi, F.K., et al. Hum. Genet. 107(1):89-91 (2000)
Schoneberg, T., et al. Biochim. Biophys. Acta 1446 (1-2), 57-70 (1999)
Marchese, A., et al. Genomics 56(1):12-21 (1999)
Immunofluorescence of purified MaxPab antibody to GPR34 on 293 cell. [antibody concentration 1 ug/ml]
Immunohistochemistry analysis in human liver tissue (formalin-fixed, paraffin-embedded) using GPR34 antibody Cat.-No. AP51926PU-N, followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPR34 antibody for IHC. Clinical relevance has not been evaluated.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane) using GPR34 antibody Cat.-No. AP51926PU-N. This demonstrates the GPR34 antibody detected the GPR34 protein (arrow).
Immunohistochemical analysis of GPR34 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of GPR34 expression in Human fetal brain (A) whole cell lysates.
Western blot analysis of extracts from K562 cells, HT-29 cells and COS-7cells, using GPR34 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of LOVO cells, using GPR34 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-GPR34 Antibody; Titration: 1.0 ug/ml; Positive Control: 293T Whole Cell
WB Suggested Anti-GPR34 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Brain
Anti-GPR34 antibody IHC of human Soft Tissue, Liposarcoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR34 antibody IHC of human Soft Tissue, Liposarcoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR34 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR34 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR34 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR34 antibody IHC of human Soft Tissue, Liposarcoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR34 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR34 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR34 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR34 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP51926PU-N | GPR34 (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
$415.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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