GCNT7 antibody
Principal name
GCNT7 antibody
Alternative names for GCNT7 antibody
C20orf105, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 7
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB)
Background of GCNT7 antibody
- Kit Component:
- - KN306375G1, Gcnt7 gRNA vector 1 in pCas-Guide vector
- KN306375G2, Gcnt7 gRNA vector 2 in pCas-Guide vector
- KN306375D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunohistochemical analysis of GCNT7 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of GCNT7 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of GCNT7 expression in HeLa (A); mouse kidney (B); rat kidney (C) whole cell lysates.
Western blot analysis of extracts from K562 cells, using GCNT7 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human ovary tissue using GCNT7 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of MCF-7 cells, using GCNT7 antibody.The picture on the right is treated with the synthesized peptide.