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GAPDH2 antibody

Principal name

GAPDH2 antibody

Alternative names for GAPDH2 antibody

GAPDHS, GAPD2, GAPDS, HSD35, HSD-35, Glyceraldehyde-3-phosphate dehydrogenase testis-specific, Spermatogenic glyceraldehyde-3-phosphate dehydrogenase, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2

SwissProt ID

O14556 (Human), Q2KJE5 (Bovin), Q64467 (Mouse)

Gene ID

26330 (GAPDHS)

Ncbi ID

NP_055179.1, 9606, NP_055179, NM_014364

Available reactivities

Hu (Human), Por (Porcine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of GAPDH2 antibody

Kit Component:: - KN205362G1, GAPDHS gRNA vector 1 in pCas-Guide vector
- KN205362G2, GAPDHS gRNA vector 2 in pCas-Guide vector
- KN205362D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

1. Tepla O. et al. In: In vitro Fertilization & Human Reproductive Genetics, ed. Monduzzi, Bologna, 107, (1999).
2. Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J.: Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization. Ceska Gynekol. 2000 Jan;65(1):28-32. Czech.
3. Peknicova J, Kyselova V, Buckiova D, Boubelik M.: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47(5):311-8.

Immunoperoxidase of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml]

H00026330-M01

Western Blot analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.

H00026330-M01

Detection limit for recombinant GST tagged GAPDHS is approximately 1ng/ml as a capture antibody.

H00026330-M01

Western Blot analysis of GAPDHS expression in transfected 293T cell line (H00026330-T02) by GAPDHS MaxPab polyclonal antibody.Lane 1: GAPDHS transfected lysate(44.50 KDa).Lane 2: Non-transfected lysate.

H00026330-D01P

Immunoprecipitation of GAPDHS transfected lysate using anti-GAPDHS MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GAPDHS MaxPab mouse polyclonal antibody (B01) (H00026330-B01).

H00026330-D01

Human Spleen; WB Suggested Anti-GAPDHS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Spleen; GAPDHS antibody - N-terminal region (ARP40187_P050) in Human Spleen cells using Western Blot

ARP40187_P050

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505454)

TA505454

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505454)

TA505454

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505454)

TA505454

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505454)

TA505454

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505454)

TA505454

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAPDHS (RC205362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAPDHS.