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Galectin-1 antibody

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Principal name

Galectin-1 antibody

Alternative names for Galectin-1 antibody

LGALS1, Galectin1, Lectin galactoside-binding soluble 1, Beta-galactoside-binding lectin L-14-I, Lactose-binding lectin 1, S-Lac lectin 1, Galaptin, 14 kDa lectin, HPL, HBL, MAPK-activating protein PM12

SwissProt ID

P09382 (Human), P11116 (Bovin), P11762 (Rat), P16045 (Mouse), P81184 (Sheep), Q49I35 (Pig)

Gene ID

3956 (LGALS1), 16852 (Lgals1), 56646

Ncbi ID

NP_002296.1, 9606, NP_032521.1, NP_002296, NP_032521, NM_002305, NM_008495, NM_019904

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Por (Porcine), Rt (Rat), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Rat, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Frozen Sections (C), Paraffin Sections (P), Flow Cytometry (F)

Background of Galectin-1 antibody

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse.[4] They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.

General readings

Okano, K., et al. Exp. Cell Res. 316(19):3282-3291(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Pal, Z., et al. J. Neuroimmunol. (2010) In press
Tang, C.E., et al. Oncol. Rep. 24(2):495-500(2010)
Garner, O.B., et al. PLoS Pathog. 6 (7), E1000993 (2010)

AP00267PU-N - Galectin-1
Western blot analysis with 250 ng His-tagged recombinant human Galectin-1 protein.
H00003956-M01 - Galectin-1
Immunofluorescence of monoclonal antibody to LGALS1 on HeLa cell. [antibody concentration 10 ug/ml]
H00003956-M01 - Galectin-1
Western Blot analysis of LGALS1 expression in transfected 293T cell line by LGALS1 monoclonal antibody (M01), clone 1E8-1B2.Lane 1: LGALS1 transfected lysate(14.7 KDa).Lane 2: Non-transfected lysate.
H00003956-M01 - Galectin-1
LGALS1 monoclonal antibody (M01), clone 1E8-1B2 Western Blot analysis of LGALS1 expression in HepG2 ( Cat # L019V1 ).
H00003956-D01P - Galectin-1
LGALS1 MaxPab rabbit polyclonal antibody. Western Blot analysis of LGALS1 expression in mouse spleen.
H00003956-D01P - Galectin-1
LGALS1 MaxPab rabbit polyclonal antibody. Western Blot analysis of LGALS1 expression in human kidney.
H00003956-D01P - Galectin-1
Western Blot analysis of LGALS1 expression in transfected 293T cell line (H00003956-T01) by LGALS1 MaxPab polyclonal antibody.Lane 1: LGALS1 transfected lysate(14.70 KDa).Lane 2: Non-transfected lysate.
H00003956-D01P - Galectin-1
LGALS1 MaxPab rabbit polyclonal antibody. Western Blot analysis of LGALS1 expression in HeLa.
AM11026SU-N - Galectin-1
Figure 1. Western blot analysis of GLT Monoclonal Antibody (Cat. #AM11026SU-N) in A375 cell line lysates. GLT (arrow) was detected using the ascites Mab. (dilution 1/1000).
AR09268PU-L - Galectin-1
AR09268PU-L - Galectin-1
Recombinant human Galectin-1, 1-135 aa: 15% SDS-PAGE (3 µg)
PAB8735 - Galectin-1
Western blot analysis of LGALS1 using 250 ng His-tagged recombinant human LGALS1.
ARP58491_P050 - Galectin-1
Mouse macrophages; LGALS1 antibody - middle region (ARP58491_P050) in Mouse macrophages cells using Western Blot
ARP58491_P050 - Galectin-1
Human Muscle; WB Suggested Anti-LGALS1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; LGALS1 antibody - middle region (ARP58491_P050) in Human Muscle cells using Western Blot
AP23675PU-N - Galectin-1
AP23675PU-N Galectin-1 antibody staining of Mouse (A) and Rat (B) Pancreas lysates and NIH3T3 (C) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
AP51860PU-N - Galectin-1
Western blot analysis of Galectin-1 Antibody Cat.-No AP51860PU-N in U251 cell line lysates (35ug/lane).
AR50602PU-N - Galectin-1
AM33389PU-S - Galectin-1
Human placenta stained with anti-Galectin-1 antibody Cat.-No AM33389PU (Clone SP247)
AM33389PU-S - Galectin-1
Western Blot analysis of NIH3T3 cell lysate with Galectin-1 antibody Cat.-No AM33389PU (Clone SP247)
AM33389PU-S - Galectin-1
Flow Cytometric analysis of Rabbit anti-Galectin-1 antibody Cat.-No AM33389PU (Clone SP247)  in HL-60 cells (green) compare to negative control of Rabbit IgG (blue)
CPA1678-100ul - Galectin-1
Western blot analysis of Galectin 1 expression in HeLa (A); HepG2 (B); Raw264.7 (C); H9C2 (D); Human liver (E) whole cell lysates.
CEK1465 - Mouse Galectin 1 ELISA Kit
Representative Standard Curve
TP304674 - Galectin-1
TP723123 - Galectin-1
TA311389 - Galectin-1
(0.5ug/ml) staining of Rat Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA311982 - Galectin-1
Western blot analysis of extracts from K562/NIH-3T3 cells, using LGALS1 antibody.The lane on the right is treated with the synthesized peptide.
TA321466 - Galectin-1
Gel: 12+15%SDS-PAGE, Lysate: 40 µg, Lane: Hela cells, Primary antibody: LGALS1 Antibody at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
TA321466 - Galectin-1
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using LGALS1 Antibody at dilution 1/25, on the right is treated with fusion protein. (Original magnification:×200)
TA321466 - Galectin-1
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using LGALS1 Antibody at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
TA321467 - Galectin-1
Predicted band size: 15 kDa . Positive control:Human fetal muscle tissue lysate. Recommended dilution: 1/500-2000. (Gel: 15%+12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
TA321467 - Galectin-1
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human cervical cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using LGALS1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x200)
TA344315 - Galectin-1
WB Suggested Anti-LGALS1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle
TA344315 - Galectin-1
Mouse macrophages
TA353837 - LGALS1
Western Blot analysis of NIH3T3 cell lysate with galectin-1 antibody
TA353837 - LGALS1
Human placenta stained with anti-galectin-1 antibody.
TA353837 - LGALS1
Flow cytometric analysis of rabbit anti-Galectin-1 (SP247) antibody in HL-60 cells (green) compare to negative control of rabbit IgG (blue).

2 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Galectin-1 (N-term) antibody
AP23675PU-N Galectin-1 antibody staining of Mouse (A) and Rat (B) Pancreas lysates and NIH3T3 (C) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt E, WB
0.1 mg / $380.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Western blotting.

Galectin-1 (100-112) antibody
  Goat Anti-Lgals1 / galectin-1 (aa100-112) Antibody  
(0.5ug/ml) staining of Rat Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / $360.00
  OriGene Technologies, Inc.

2 Item(s)

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