GADD45A antibody
Principal name
GADD45A antibody
Alternative names for GADD45A antibody
GADD45, DDIT1, DDIT-1, Growth arrest and DNA damage-inducible protein GADD45 alpha, DNA damage-inducible transcript 1 protein
Ncbi ID
9606, NP_001915, NM_001924, NP_001915.1, NP_031862.1, NM_007836
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of GADD45A antibody
- Kit Component:
- - KN204005G1, GADD45A gRNA vector 1 in pCas-Guide vector
- KN204005G2, GADD45A gRNA vector 2 in pCas-Guide vector
- KN204005D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of anti-GADD45A Antibody (N-term) (Cat.#AP12434PU-N) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.
Western blot analysis of GADD45A Antibody (C-term) (AP18028PU-N) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.
Recombinant human GADD45A, 1-165 aa, His-tagged: 15% SDS-PAGE (3 ug)
Human Heart; WB Suggested Anti-GADD45A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human heart; GADD45A antibody - C-terminal region (ARP60167_P050) in Human Heart cells using Western Blot
Western Blot analysis of GADD45A expression in transfected 293T cell line (H00001647-T01) by GADD45A MaxPab polyclonal antibody.Lane 1: GADD45A transfected lysate(18.30 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP2K6 and GADD45A HeLa cells were stained with anti-MAP2K6 rabbit purified polyclonal 1:1200 and anti-GADD45A mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (RC204005, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (RC204005, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.
WB Suggested Anti-GADD45A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human heart
Western blot detection of GADD45 a in CHO-K1 cell lysate(B)and CHO-K1 transfected by GADD45 a His fusion protein(A)cell lysate using GADD45 a mouse mAb (1:1000 diluted). Predicted band size:22KDa. Observed band size:22KDa.
Western blot analysis of extracts of various cell lines, using GADD45A antibody.
Immunohistochemistry of paraffin-embedded human esophagus using GADD45A antibody at dilution of 1:100 (400x lens).
The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (GADD45A Antibody) at dilution 1/30. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA332434 | GADD45A antibody |
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| Rabbit anti-GADD45A Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
$335.00
|
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA350003 | GADD45A antibody |
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| Rabbit Polyclonal Anti-GADD45A Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP12434PU-N | GADD45A (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
$415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP18028PU-N | GADD45A (C-term) antibody |
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 |
Rabbit | Hu | WB |
0.4 ml /
$415.00
|
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| OriGene Technologies GmbH | |||||||