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GABRA2 antibody

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Principal name

GABRA2 antibody

Alternative names for GABRA2 antibody

GABRA-2, GABA A receptor subunit alpha-2, Gamma-aminobutyric acid receptor subunit alpha-2

SwissProt ID

P10063 (Bovin), P23576 (Rat), P26048 (Mouse), P47869 (Human)

Gene ID

2555 (GABRA2), 289606 (Gabra2), 14395

Ncbi ID

NP_000798, ADZ15724.1, 10116, NP_032092.1, NP_000798.2, NM_008066, NM_001114175

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)

Background of GABRA2 antibody

GABA (?-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake and metabolism occur in the nervous system.The GABA transmitter interacts with two major types of receptors: ionotropic GABAA (GABAAR) and the metabotropic GABAB) receptors. The GABAAR belong to the ligand gated ion channel superfamily.The GABAAR, is a heteropentamer, with all of its five subunits contributing to the pore formation. To date, eight subunit isoforms were cloned: a, Ã?, ?, d, e, p, ?, and ?. Six a subunit isoforms were described to exist in mammals (a1-a6). The tive GABAA receptor, in most cases, consists of 2a, 2Ã? and 1? submit. The a subunit is the most common subunit and is expressed ubiquitously. It determines the affinities for allosteric ligands shown by GABAAR.The a2-subunit mR is strongly expressed only in the hippocampus and the hypothalamus. It occurs very early in rat brain development and declines in adulthood. The failure to complete the normal transition between the a-subunits that are highly expressed in early development (a2, a3, a5) to those expressed in adulthood (a1) might play a major role in the development of temporal lobe epilepsy.

General readings

Hadingham, K.L., et al., (1993) Mol. Pharmacol. 43:970-975.

AVARP13026_T100 - GABRA2
Human HepG2; WB Suggested Anti-GABRA2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; GABRA2 antibody - middle region (AVARP13026_T100) in Human HepG2 cells using Western Blot
TA309125 - GABRA2
Western blot of 10 µg of rat cortex lysate showing immunolabeling of the ~53k a2-subunit of the GABAA-R.
TA309126 - GABRA2
Western blot of mouse brain lysates from wild type (Control) and α2-knockout (α2-K/O) animals showing specific immunolabeling of the ~51k α2-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α2-knockout animals.
TA325099 - GABRA2
GABRA2 Antibody (C-term) (Cat.# TA325099) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GABRA2 antibody detected the GABRA2 protein (arrow).
TA325099 - GABRA2
GABRA2 Antibody (C-term) (Cat. #TA325099) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GABRA2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
TA325099 - GABRA2
GABRA2 Antibody (C-term) (Cat. #TA325099) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA327182 - GABRA2
Western blot analysis of extracts of various cell lines, using GABRA2 antibody.
TA327182 - GABRA2
Immunohistochemistry of paraffin-embedded rat kidney using GABRA2 antibody at dilution of 1:100 (200x lens).
TA327182 - GABRA2
Immunohistochemistry of paraffin-embedded human esophageal cancer using GABRA2 antibody at dilution of 1:100 (400x lens).
TA328810 - GABRA2
Western blot analysis of rat brain membranes: 1. Anti-GABA(A) a2 Receptor antibody, (1:200). 2. Anti-GABA(A) a2 Receptor antibody, preincubated with the control peptide antigen.
TA328810 - GABRA2
Expression of GABA(A) a2 Receptor in mouse cerebellum. Immunohistochemical staining of mouse formalin-fixed frozen cerebellum sections with Anti-GABA(A) a2 Receptor antibody. The receptor subunit is localized mainly on the soma of Purkinje cells. Staining with mouse anti-parvalbumin (red) suggests that GABA(A) a2 Receptor subunit is restricted to a sub-region of the Purkinje cell.
TA330434 - GABRA2
WB Suggested Anti-GABRA2 Antibody Titration: 1.25ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate
CPA9288-100ul - GABRA2
Western blot analysis of GABRA2 expression in mouse brain (A); rat brain (B) whole cell lysates.
CPA9288-100ul - GABRA2
Immunohistochemical analysis of GABRA2 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

3 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GABRA2 antibody
  Rabbit polyclonal GABRA2 Antibody (C-term)  
GABRA2 Antibody (C-term) (Cat.# TA325099) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GABRA2 antibody detected the GABRA2 protein (arrow). Rabbit IgG Purified Bov, Hu, Ms, Rt F, P, WB
0.4 ml / $415.00
  OriGene Technologies, Inc.
+2 additional images

GABRA2 antibody
  GABRA2 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using GABRA2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / $335.00
  OriGene Technologies, Inc.
+2 additional images

GABRA2 antibody
  Rabbit Polyclonal Anti-GABA (A) alpha2 Receptor  
Western blot analysis of rat brain membranes: 1. Anti-GABA(A) a2 Receptor antibody, (1:200). 2. Anti-GABA(A) a2 Receptor antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / $585.00
  OriGene Technologies, Inc.
+1 additional image

3 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (3)
Clonality
  1. Polyclonal (3)
Target
  1. GABRA2 (3)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (3)
  3. Rat (Rt) (3)
  4. Bovine (Bov) (1)
Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Flow cytometry (F) (1)
  3. Paraffin Sections (P) (1)
Presentation
  1. Purified (3)
Host
  1. Rabbit (3)
Images
  1. 1 image (3)
  2. 3+ images (2)
Images: Applications
  1. IHC (3)
  2. Western blot / Immunoblot (WB) (3)
Manufacturer
  1. OriGene Technologies (3)
Service
 
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