Fibulin-4 antibody
Principal name
Fibulin-4 antibody
Alternative names for Fibulin-4 antibody
Fibulin 4, EFEMP2, FBLN4, UNQ200/PRO226, Protein UPH1, EGF-containing fibulin-like extracellular matrix protein 2
Ncbi ID
NP_058634.4, NP_058634, NP_067449.3, NM_001164352, NM_016938
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E)
Background of Fibulin-4 antibody
- Kit Component:
- - KN305035G1, Efemp2 gRNA vector 1 in pCas-Guide vector
- KN305035G2, Efemp2 gRNA vector 2 in pCas-Guide vector
- KN305035D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Chen,Q. et.al., Biochem. J. 423 (1): 79-89 (2009)
Choudhury,R. et.al., J. Biol. Chem. 284 (36): 24553-24567 (2009)
El-Hallous,E. et.al., J. Biol. Chem. 282 (12): 8935-8946 (2007)
Western Blot analysis of EFEMP2 expression in transfected 293T cell line (H00030008-T01) by EFEMP2 MaxPab polyclonal antibody.Lane 1: EFEMP2 transfected lysate(48.73 KDa).Lane 2: Non-transfected lysate.
EFEMP2 MaxPab polyclonal antibody. Western Blot analysis of EFEMP2 expression in human kidney.
FACS analysis of negative control 293 cells (Black) and EFEMP2 expressing 293 cells (Green) using EFEMP2 purified MaxPab mouse polyclonal antibody.
Western Blot analysis of EFEMP2 expression in transfected 293T cell line (H00030008-T01) by EFEMP2 MaxPab polyclonal antibody.Lane 1: EFEMP2 transfected lysate(48.73 KDa).Lane 2: Non-transfected lysate.
EFEMP2 MaxPab polyclonal antibody. Western Blot analysis of EFEMP2 expression in human kidney.
FACS analysis of negative control 293 cells (Black) and EFEMP2 expressing 293 cells (Green) using EFEMP2 purified MaxPab mouse polyclonal antibody.
Western Blot analysis of EFEMP2 expression in transfected 293T cell line (H00030008-T01) by EFEMP2 MaxPab polyclonal antibody.Lane 1: EFEMP2 transfected lysate(49.40 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry (IHC) analyzes of Fibulin-4 antibody (Cat.-No.: AP01398PU-N) in paraffin-embedded human placenta tissue.
Western blot (WB) analysis of Fibulin-4 antibody (Cat.-No.: AP01398PU-N) in extracts from K562 cells.
Immunoprecipitation of EFEMP2 transfected lysate using anti-EFEMP2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with EFEMP2 MaxPab mouse polyclonal antibody (B01) (H00030008-B01).
Human NCI-H226; WB Suggested Anti-EFEMP2 Antibody Titration: 0.2-1 ug/ml. Positive Control: NCI-H226 cell lysate; EFEMP2 antibody - middle region (ARP55351_P050) in Human NCI-H226 cells using Western Blot
Western blot analysis of Fibulin-4 (arrow) in HepG2 cell line lysates (35ug/lane) using Fibulin-4 antibody Cat.-No. AP51375PU-N (C-term).
Western blot analysis of Fibulin-4(arrow) in mouse NIH-3T3 cell line lysates (35ug/lane) using Fibulin-4 antibody Cat.-No. AP51375PU-N (C-term).
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram) using Fibulin-4 antibody Cat.-No. AP51375PU-N (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
Immunohistochemical analysis of Fibulin 4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Fibulin 4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Fibulin 4 expression in SW480 (A); HepG2 (B) whole cell lysates.
Western blot analysis of extracts from K562 cells and 3T3 cells, using EFEMP2 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using EFEMP2 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using EFEMP2 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-EFEMP2 Antibody Titration: 0.2-1 ug/ml; Positive Control: NCI-H226 cell lysate
Western blot analysis of EFEMP2 expression in 3T3 cells
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315408 | Fibulin-4 antibody |
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| Rabbit polyclonal anti-EFEMP2 antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, ICC/IF, P, WB |
0.1 ml /
$335.00
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| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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