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FBXO43 antibody

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Principal name

FBXO43 antibody

Alternative names for FBXO43 antibody

F-box only protein 43, EMI2, FBP5B, Endogenous meiotic inhibitor 2

SwissProt ID

Q4G163 (Human), Q66H04 (Rat), Q8AXF4 (Xenla), Q8CDI2 (Mouse)

Gene ID

286151 (FBXO43), 100101275 (fbxo43)

Ncbi ID

NP_001025031.2, 9606, NP_001093338.1, XP_209918, NM_001029860, AF029213

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)

Background of FBXO43 antibody

Kit Component:
- KN216347G1, FBXO43 gRNA vector 1 in pCas-Guide vector
- KN216347G2, FBXO43 gRNA vector 2 in pCas-Guide vector
- KN216347D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature 1997;388:548-54
2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an IkappaB kinase. Cell 1997;90:373-83
3. Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation. Cell 1997;91:243-52
Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IkappaB kinase-alpha: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK. Science 1997;278:866-9

AP07584PU-N - FBXO43
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Liver.
PAB10206 - FBXO43
Western blot using FBXO43 polyclonal antibody ( Cat # PAB10206 ).Panels A and B show two identical membranes containing marker, in vitro translated FBXO43 ( FBXO43 ivt ), and a whole cell extract from 293T cells loaded in duplicate.Panel A shows antibody reactivity.Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide.This pre-incubation greatly diminishes specific band recognition.Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells.When this experiment was repeated using a mock in vitro translation, no band was detected ( data not shown ).Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute.
TA306000 - FBXO43
Western blot analysis of IL-1RAcP in HeLa whole cell lysate with IL-1RAcP antibody (C2) at 1:1000 dilution.
TA306001 - FBXO43
Western blot analysis of IL-1RAcP in HeLa whole cell lysate with IL-1RAcP antibody at 1:500 dilution.
TA306001 - FBXO43
Immunocytochemistry of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 5 ug/mL.
TA308747 - FBXO43
Sample (30 ug of whole cell lysate). A: NIH-3T3. B: JC. 7.5% SDS PAGE. TA308747 diluted at 1:1000.
TA308747 - FBXO43
Sample (30 ug of whole cell lysate). A: 293T. 7.5% SDS PAGE. TA308747 diluted at 1:1000.
TA308747 - FBXO43
Immunohistochemical analysis of paraffin-embedded Saos-2 xenograft, using FBXO43 (TA308747) antibody at 1:100 dilution.
TA308747 - FBXO43
Immunofluorescence analysis of paraformaldehyde-fixed A431, using FBXO43(TA308747) antibody at 1:200 dilution.
TA319329 - FBXO43
WB using Anti-Fbp5B antibody. Panels A and B show two identical membranes containing marker, in vitro translated Fbp5b (Fbp5b ivt), and a whole cell extract from 293T cells loaded in duplicate). Panel A shows antibody reactivity. Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FBXO43 (N-term) antibody
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Liver. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / $425.00
  OriGene Technologies GmbH

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  2. Mouse (Ms) (1)
  3. Rat (Rt) (1)
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  1. Enzyme Immunoassay (E) (1)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  3. Paraffin Sections (P) (1)
  4. Western blot / Immunoblot (WB) (1)
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