FANCD2 antibody
Principal name
FANCD2 antibody
Alternative names for FANCD2 antibody
Fanconi anemia group D2 protein, FACD2, FACD
Ncbi ID
NP_001018125.1, NP_001018125, 9606, NP_149075.2, NP_001028416.2
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of FANCD2 antibody
Fanconi Anemia Complementation Group D2 (FANCD2) protein is one of at least six factors shown to be involved in the autosomal-recessive cancer-prone disorder, Fanconi Anemia (FA). FA group D has been shown to be comprised of two separate proteins, FANCD1 and FANCD2. Mutations in BRCA2 can cause FANCD1. FANCD2 has been shown to colocalize with BRCA1 in ionizing radiation-induced foci. FANCD2 is involved in genomic resistance to DNA cross-linking reagents, and the arrest of DNA synthesis following exposure to ionizing radiation. FANCD2 has been shown to directly interact with NBS1.
FANCD2 is one of at least four different genes, of which when mutated can cause the Fanconi anemia phenotype characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomcyin C. FANCD2 is phosphorylated by ATM following ionizing radiation and this phosphorylation is required for activation of an S-phase checkpoint. It is also monoubiquitinated in response to DNA damage. It is postulated that FANCD2 may have a role in the cellular response to stalled replication forks or in the repair of replication-associated double-strand breaks.
Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using FANCD2 antibody (Cat.-No. AP06115PU-N).
Western blot (WB) analysis of FANCD2 antibody (Cat.-No.: AP06115PU-N) at 1/500 dilution
Lane1:Hela cell lysate
Lane2:SP2/0 cell lysate
Lane3:Mouse liver tissue lysate
Lane1:Hela cell lysate
Lane2:SP2/0 cell lysate
Lane3:Mouse liver tissue lysate
Formalin-Fixed Paraffin-Embedded Human Liver stained with FANCD2 antibody AM06065PU-N at 2.5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Expression of FancD2 ( mAb ) in different cell lines : proliferating cells were treated with IR or kept as mock of IR. Eight hours after IR, local proteins were isolated for Western blot analysis. Fifty microgram protein was loaded each lane. Working dilution of FANCD2 monoclonal antibody, clone FI-17 ( Cat # MAB2398 ) is 1 : 1000. Lane1 : U2OS, Lane2 : U2OS+IR, Lane3 and 4 : MEF, Lane5 : MO59K+IR, Lane6 : SiHa+IR.
Staining of human prostate, glandular epithelium using FANCD2 polyclonal antibody ( Cat # PAB12428 ).
FANCD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h. FANCD2 were stained with FANCD2 polyclonal antibody ( Cat # PAB12428 ).
FANCD2 polyclonal antibody ( Cat # PAB12428 ) was used to stain FANCD2. FANCD2 colocalizes in vivo with another protein in U-2 OS cells after cell exposure to IR. Proliferating U-2 OS cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h.
Western blot analysis of FANCD2 in HeLa whole cell extract using FANCD2 polyclonal antibody ( Cat # PAB12428 ).
FA-D2 fibroblasts were stably transfected with either pMMP ( empty vector, A ), FANCD2 ( wt, B ), FANCD2 ( S222A, C ), FANCD2 ( triple mutant, D ), or FANCD2 ( quadruple mutant, E ). Cells were irradiated with 15 Gy ( + ), and whole cell lysates were immunoblotted with either anti-FANCD2 or Phospho-FANCD2 S222 polyclonal antibody, ( Cat # PAB12530 ).
Indicated lymphoblasts ( PD7, WT : GM1526, AT ) were irradiated with 15 Gy ( 2 ), and immunoblotted with anti-FANCD2 and FANCD2 (phospho S222) polyclonal antibody ( Cat # PAB12530 ).
Western blot analysis of extracts from HT29 cells treated with CalyculinA using Phospho-FANCD2 (Ser222) antibody AP55916PU-N.The lane on the right is treated with the antigen-specific peptide.
ELISA: FANCD2 Antibody
Western Blot: FANCD2 Antibody (FI-17) - Expression of FancD2 (mAb) in different cell lines. Proliferating cells were treated with 10-Gy IR. Fifty microgram protein was loaded each lane. Working dilution - 1:1000 LCL 6% SDS-PAGE.
Western Blot: FANCD2 Antibody (FI-17) - Western blot of FANCD2 on MCF7 cell line (lane 1) and HeLa whole cell extract (lane 2) using anti-FANCD2.
Western blot analysis of extracts from JK cells, using FANCD2 (Ab-691) antibody.The lane on the right is treated with the synthesized peptide.
Predicted band size: 166 kDa . Positive control: HL-60 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of FANCD2 expression in CalyculinA treated HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts of various cell lines, using FANCD2 antibody.
Immunofluorescence analysis of MCF7 cell using FANCD2 antibody. Blue: DAPI for nuclear staining.
Western Blot: FANCD2 Antibody [TA336272] - Analysis of FANCD2 in human PD20 cells. Image courtesy of anonymous customer review
Western Blot: FANCD2 Antibody [TA336272] - FANCD2 in HeLa WCE using NB 100-182 (lot C).
Immunohistochemistry: FANCD2 Antibody [TA336272] - Staining of human prostate, glandular epithelium.
Immunocytochemistry/Immunofluorescence: FANCD2 Antibody [TA336272] - FancD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double -color immunofluorescence stainin
Immunocytochemistry/Immunofluorescence: FANCD2 Antibody [TA336272] - FancD2 colocalizes in vivo with another protein in U2OS cells after cell exposure to IR. Proliferating U2OS cells were exposed to 10 Gy of IR and double -color immunofluorescence stainin
Western Blot: FANCD2 [p Ser222] Antibody [TA336310] - FA-D2 fibroblasts were stably transfected with either pMMP (empty vector, A), FANCD2 (wt, B), FANCD2 (S222A, C), FANCD2 (triple mutant, D), or FANCD2 (quadruple mutant, E).
Western Blot: FANCD2 [p Ser222] Antibody [TA336310] - Indicated lymphoblasts (PD7, WT: GM1526, AT) were irradiated with 15 Gy (2), and immunoblotted with anti-FANCD2 and anti-FANCD2 (pS-222)
Simple Western: FANCD2 Antibody [TA336730] - Simple Western lane view shows a specific band detected for FANCD2 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa).
Western Blot: FANCD2 Antibody [TA336730] - Cell lysates of wildtype lymphoblasts (HSC93) and fibroblasts (LN9SV) and FANCD2 mutated lymphoblasts (VU1288) and (PD20) probed with anti-FANCD2 antibody showing clear FANCD2 protein bands in the wild-type cells
Western Blot: FANCD2 Antibody [TA336730] - Immunofluorescence with anti-FANCD2, polyclonal(A-C) triple staining of the adult stomach with DAPI(blue), anti-FANCD2(red), and anti Ki-67 (green), (D) Merged image of FANCD2 and Ki-67 staining showing co-expres
Immunohistochemistry: FANCD2 Antibody [TA336730] - Immunohistochemical analysis of FANCD2 expression in FA cell lines with anti-FANCD2, polyclonal FANCD2-expressing cells exhibit dark brown nuclear staining: cell types are specified. Negative controls wit
Western blot analysis of FANCD2 phosphorylation expression in CalyculinA treated HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
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| TA336272 | FANCD2 antibody |
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| Rabbit Polyclonal FANCD2 Antibody | |||||||
![Western Blot: FANCD2 Antibody [TA336272] - Analysis of FANCD2 in human PD20 cells. Image courtesy of anonymous customer review](https://m1.acris-antibodies.com/catalog/product/cache/2/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB100-182-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Prim | C, ICC/IF, IP, P, WB |
50 µl /
$475.00
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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